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Journal of Clinical Microbiology, September 1998, p. 2748-2751, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Discrimination of Burkholderia gladioli from Other Burkholderia Species Detectable in Cystic Fibrosis Patients by PCR

Adolf Bauernfeind,* Ines Schneider, Renate Jungwirth, and Carsten Roller

Department of Experimental Medical Microbiology and Cystic Fibrosis, Max von Pettenkofer Institute, D-80336 Munich, Germany

Received 31 January 1998/Returned for modification 2 April 1998/Accepted 11 June 1998

A procedure for molecular identification of Burkholderia gladioli is described. Specific 16S and 23S rRNA gene signature sequences were defined as primers for PCR. The method allows rapid and specific discrimination of B. gladioli from related species (B. cepacia, B. multivorans, B. vietnamiensis, B. mallei, B. pseudomallei, Ralstonia pickettii, and R. eutropha) and should contribute to the clarification of its role as a human pathogen, e.g., in cystic fibrosis.

* Corresponding author. Mailing address: Department of Experimental Medical Microbiology and Cystic Fibrosis, Max-von-Pettenkofer-Institute, Pettenkoferstraße 9a, D-80336 München, Germany. Phone: 0049-89-5160-5268. Fax: 0049-89-5160-5266. E-mail: Adolf.Bauernfeind{at}mvp-bak.med.uni-muenchen.de.

Journal of Clinical Microbiology, September 1998, p. 2748-2751, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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