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Journal of Clinical Microbiology, September 1998, p. 2752-2754, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Novel Method for Rapid Measurement of Growth of Mycobacteria in Detergent-Free Media

Paul R. Meyers,1 William R. Bourn,2 Lafras M. Steyn,1 Paul D. van Helden,2 Albert D. Beyers,2 and Gordon D. Brown2,*

Department of Medical Microbiology, University of Cape Town, Observatory, Cape Town 7925,1 and MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, University of Stellenbosch, Tygerberg, 7505,2 South Africa

Received 16 March 1998/Returned for modification 12 May 1998/Accepted 1 June 1998

We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for monitoring mycobacterial growth but is particularly useful for heavily clumped cultures grown in defined minimal medium.

* Corresponding author. Mailing address: Department of Medical Biochemistry, P.O. Box 19063, Tygerberg Medical School, Tygerberg, 7505 South Africa. Phone: 27 21 938-9402/8. Fax: 27 21 931-7810. E-mail: gbro{at}gerga.sun.ac.za.

Journal of Clinical Microbiology, September 1998, p. 2752-2754, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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