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Journal of Clinical Microbiology, September 1998, p. 2759-2762, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Epidemiology of Extended-Spectrum beta -Lactamase-Producing Klebsiella pneumoniae Isolates in a District Hospital in Taiwan

Peter Yuk-Fong Liu,1,* Jai-Chin Tung,1 Se-Chin Ke,2 and Shun-Liang Chen2

Department of Internal Medicine1 and Laboratory of Clinical Microbiology,2 Shalu Tungs' Memorial Hospital, Shalu, Taiwan, Republic of China

Received 9 February 1998/Returned for modification 25 March 1998/Accepted 15 June 1998

Thirty-one of 104 clinical isolates of Klebsiella pneumoniae collected over a period of 8 months were found to be putative extended-spectrum beta -lactamase (ESBL) producers. Isoelectric focusing and an iodine overlay agar method were used for preliminary identification of the ESBLs. They were further identified by DNA sequencing. Seventy-one percent of the isolates were found to produce SHV-5. The variation in the ESBL patterns of these isolates was slight, with only five patterns being identified. The strains were typed by pulsed-field gel electrophoresis (PFGE), and 16 different genotypes were identified. When the PFGE patterns were analyzed by the algorithmic clustering method called the unweighted-pair group method using arithmetic averages, five clusters were found. However, significant genetic variations were found among 11 isolates and between each cluster. A plasmid of 36 kb was found in all clinical isolates and in the transconjugants. Our results indicate that the increase in the number of ESBL-producing K. pneumoniae isolates in this hospital is due mainly to the dissemination of a resistance plasmid rather than to the clonal spread of a few epidemic strains.

* Corresponding author. Mailing address: Number 30, Ching-Cheng 20th St., Taichung City, Taiwan, Republic of China. Phone and fax: 886-4-4632425. E-mail: liu712{at}top2.ficnet.net.tw.

Journal of Clinical Microbiology, September 1998, p. 2759-2762, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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