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Journal of Clinical Microbiology, September 1998, p. 2769-2771, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cord Formation in BACTEC Medium Is a Reliable, Rapid Method for Presumptive Identification of Mycobacterium tuberculosis Complex

Yvette S. McCarter,1,2,* Irene N. Ratkiewicz,1 and Ann Robinson1,2

Division of Microbiology, Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, Connecticut 06102-5037,1 and Department of Laboratory Medicine, University of Connecticut School of Medicine, Farmington, Connecticut2

Received 27 February 1998/Returned for modification 6 April 1998/Accepted 10 June 1998

Serpentine cord formation in BACTEC 12B medium was evaluated as a rapid method for the presumptive identification of M. tuberculosis complex. Kinyoun acid-fast stained smears were prepared from 666 positive BACTEC 12B bottles and examined for the presence or absence of serpentine cording. Cord formation had a sensitivity, specificity, positive predictive value, and negative predictive value of 89.2, 99.2, 98.5, and 94.2%, respectively. The evaluation of the presence of cord formation in BACTEC 12B medium is reliable and permits the rapid presumptive reporting of M. tuberculosis.

* Corresponding author. Mailing address: Division of Microbiology, Department of Pathology and Laboratory Medicine, Hartford Hospital, 80 Seymour St., Box 5037, Hartford, CT 06102-5037. Phone: (860) 545-2242. Fax: (860) 545-5206. E-mail: ymccart{at}harthosp.org.

Journal of Clinical Microbiology, September 1998, p. 2769-2771, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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