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Journal of Clinical Microbiology, September 1998, p. 2772-2774, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Helicobacter pylori in Stool Specimens by PCR and Antigen Enzyme Immunoassay

Athanasios Makristathis,1,* Eva Pasching,1 Kurt Schütze,2 Margit Wimmer,1 Manfred L. Rotter,1 and Alexander M. Hirschl1

Department of Clinical Microbiology, Hygiene Institute of the University of Vienna,1 and Medical Department I, Hanusch Hospital,2 Vienna, Austria

Received 21 January 1998/Returned for modification 16 March 1998/Accepted 11 June 1998

A highly sensitive seminested PCR assay to detect Helicobacter pylori DNA in feces was developed. PCR with stool specimens and a novel antigen enzyme immunoassay (EIA) for H. pylori detection in feces were evaluated as diagnostic tools and in follow-up with samples from 63 infected and 37 noninfected persons. Infected individuals received eradication therapy followed by endoscopic follow-up 35 days after the start of treatment. At that time, a second stool specimen was obtained from 55 of these patients. Before eradication, the sensitivity of PCR was 93.7% and that of EIA 88.9%. Specificities were 100 and 94.6%, respectively. Of the 55 follow-up specimens, 41 originated from patients from whom H. pylori had been eradicated. Of these, 21 were still positive by PCR and 13 were positive by EIA, indicating that 1 month may be too short a period for follow-up evaluation of stool specimens by these tests.

* Corresponding author. Mailing address: Department of Clinical Microbiology, University Hospital of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. Phone: 43 (1) 40400-5157. Fax: 43 (1) 40400-5162. E-mail: Athanasios.Makristathis{at}akh-wien.ac.at.

Journal of Clinical Microbiology, September 1998, p. 2772-2774, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.