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Journal of Clinical Microbiology, September 1998, p. 2778-2781, Vol. 36, No. 9
Department of Veterinary Pathobiology,
College of Veterinary Medicine, Texas A&M University, College
Station, Texas1;
Department of
Microbiology, University of Toronto, and Mount Sinai and Princess
Margaret Hospitals, Toronto, Ontario,
Canada2; and
Department of Clinical
Microbiology, The Hebrew University- Hadassah Medical School,
Jerusalem, Israel3
Received 17 February 1998/Returned for modification 22 April
1998/Accepted 22 June 1998
Streptococcus iniae is a cause of septicemia,
meningoencephalitis, and death in farmed fish and of cellulitis in
human beings. A set of nested oligonucleotide PCR primers that
specifically amplified a 373-bp subunit from a variety of clinical
isolates from farmed fish and human patients were constructed from a
524-bp consensus sequence of the S. iniae 16S-23S ribosomal
DNA intergenic spacer.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Development of Specific Nested Oligonucleotide PCR
Primers for the Streptococcus iniae 16S-23S Ribosomal
DNA Intergenic Spacer
*
Corresponding author. Mailing address: Department of
Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M
University, College Station, TX 77843. Phone: (409) 845-5066. Fax:
(409) 862-6682. E-mail: bberridge{at}cvm.tamu.edu.
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