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Journal of Clinical Microbiology, September 1998, p. 2778-2781, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of Specific Nested Oligonucleotide PCR Primers for the Streptococcus iniae 16S-23S Ribosomal DNA Intergenic Spacer

Brian R. Berridge,1,* Jeffrey D. Fuller,2 Joyce de Azavedo,2 Donald E. Low,2 Herve Bercovier,3 and Paul F. Frelier1

Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas1; Department of Microbiology, University of Toronto, and Mount Sinai and Princess Margaret Hospitals, Toronto, Ontario, Canada2; and Department of Clinical Microbiology, The Hebrew University- Hadassah Medical School, Jerusalem, Israel3

Received 17 February 1998/Returned for modification 22 April 1998/Accepted 22 June 1998

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843. Phone: (409) 845-5066. Fax: (409) 862-6682. E-mail: bberridge{at}cvm.tamu.edu.

Journal of Clinical Microbiology, September 1998, p. 2778-2781, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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