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Journal of Clinical Microbiology, January 1999, p. 14-17, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of PCR, Culture, and Serological Tests for Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infection in Children

J. W. Dorigo-Zetsma,1,* S. A. J. Zaat,1 P. M. E. Wertheim-van Dillen,1 L. Spanjaard,1 J. Rijntjes,2 G. van Waveren,3 J. S. Jensen,4 A. F. Angulo,5 and J. Dankert1

Laboratory of Medical Microbiology1 and Department of Paediatrics,2 Academic Medical Center, and Department of Paediatrics, Boven IJ Hospital,3 Amsterdam, and Laboratory for Control of Biological Products, Mycoplasma Department, National Institute of Public Health and the Environment, Bilthoven,5 The Netherlands, and Mycoplasma Laboratory, Neisseria Department, Statens Serum Institut, Copenhagen, Denmark4

Received 29 June 1998/Returned for modification 20 August 1998/Accepted 13 October 1998

For diagnosis of Mycoplasma pneumoniae infection we compared two rapid tests, PCR and the immunoglobulin M immunofluorescence assay (IgM IFA), with culture and the complement fixation test (CFT), in a prospective study among 92 children with respiratory tract infection and 74 controls. Based on positivity of culture and/or CFT as the diagnostic criterion, nine patients (10%) were diagnosed with M. pneumoniae infection. All patients positive by culture were also positive by PCR. In all controls cultures, PCRs, and serological assays were negative, except in one with a positive IgM IFA. The IgM IFA had a low positive predictive value of 50%. Only a combination of PCR (seven patients) and CFT (seven patients) allowed diagnosis of all cases.

* Corresponding author. Present address: Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31-30-2743705. Fax: 31-30-2744449. E-mail: Wendelien.Dorigo{at}rivm.nl.

Journal of Clinical Microbiology, January 1999, p. 14-17, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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