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Journal of Clinical Microbiology, January 1999, p. 157-160, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Penicillin Susceptibility in Streptococcus pneumoniae by pbp2b PCR-Restriction Fragment Length Polymorphism Analysis

A. M. O'Neill, S. H. Gillespie,* and G. C. Whiting

Department of Medical Microbiology, Royal Free Hospital School of Medicine, London NW3 2PF, United Kingdom

Received 18 June 1998/Returned for modification 4 August 1998/Accepted 29 September 1998

A PCR-restriction fragment length polymorphism strategy directed against the pbp2b gene was evaluated for identification of penicillin susceptibility. A total of 106 United Kingdom (U.K.), 30 Danish, and 11 Papua New Guinean strains were tested. Of the U.K. strains, all the susceptible and all but one of the resistant isolates were correctly assigned. By using conventional definitions of "not resistant" and "not susceptible," the sensitivities were 97.5 and 94.4%, the specificities were 100 and 98.9%, the positive predictive values were 100 and 94.4%, and the negative predictive values were 93.1 and 98.9%, respectively. This technique may allow susceptible (MIC, <0.1 mg/liter) and resistant (MIC, >1 mg/liter) isolates to be distinguished in a single PCR.

* Corresponding author. Mailing address: Department of Medical Microbiology, Royal Free Hospital School of Medicine, Rowland Hill St., London NW3 2PF, United Kingdom. Phone: 44-(0)171-794-0500. Fax: 44-(0)171-794-0433. E-mail: stepheng{at}rfhsm.ac.uk.

Journal of Clinical Microbiology, January 1999, p. 157-160, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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