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Journal of Clinical Microbiology, January 1999, p. 206-208, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Two PCRs for Detection of Mycobacterium ulcerans

A. Guimaraes-Peres,1 F. Portaels,1 P. de Rijk,1 K. Fissette,1 S. R. Pattyn,1 J.-P. van Vooren,2 and P.-A. Fonteyne1,*

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp,1 and Department of Pneumology, Erasme University Hospital, Brussels,2 Belgium

Received 15 June 1998/Returned for modification 2 August 1998/Accepted 13 October 1998

Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.

* Corresponding author. Mailing address: Institute of Tropical Medicine, Department of Microbiology, Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: 32 3 247 63 36. Fax: 32 3 247 63 33. E-mail: fonteyne{at}itg.be.

Journal of Clinical Microbiology, January 1999, p. 206-208, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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