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Journal of Clinical Microbiology, January 1999, p. 209-210, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identity of Ehrlichial DNA Sequences Derived from Ixodes ricinus Ticks with Those Obtained from Patients with Human Granulocytic Ehrlichiosis in Slovenia

M. Petrovec,^1,* J. W. Sumner,² W. L. Nicholson,² J. E. Childs,² F. Strle,³ J. Barli,¹ S. Lotri-Furlan,¹ and T. Avi upanc¹

Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana,¹ and Department of Infectious Diseases, University Medical Center,³ Ljubljana, Slovenia, and Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia²

Received 25 June 1998/Returned for modification 7 August 1998/Accepted 2 October 1998

Adult Ixodes ricinus (Acari: Ixodidae) ticks collected near Ljubljana, Slovenia, were tested for the agent of human granulocytic ehrlichiosis (HGE) by using PCR assays based on the 16S rRNA gene. Three (3.2%) of 93 ticks were found to contain granulocytic ehrlichiae. Nucleotide sequences of portions of the bacterial groESL heat shock operon amplified from these ticks were identical or nearly (99.8%) identical to those previously determined for human patients with HGE from Slovenia, providing additional evidence that the ticks were infected with the HGE agent. This study identified I. ricinus as the likely vector for these ehrlichial pathogens of humans in this part of Europe.

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^* Corresponding author. Mailing address: Institute of Microbiology and Immunology, Zaloka 4, 1105 Ljubljana, Slovenia. Phone: 386 61 316 593. Fax: 386 61 302 895. E-mail: petrovec{at}mf.uni-lj.si.

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Journal of Clinical Microbiology, January 1999, p. 209-210, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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