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Journal of Clinical Microbiology, January 1999, p. 218-220, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison between PCR and Detection of Antigen in Sera for Diagnosis of Pulmonary Aspergillosis

Sumio Kawamura, Shigefumi Maesaki, Tetsuhiro Noda, Youichi Hirakata, Kazunori Tomono, Takayoshi Tashiro, and Shigeru Kohno*

Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan

Received 8 April 1998/Returned for modification 26 May 1998/Accepted 16 October 1998

We evaluated the usefulness of PCR and antigen detection for the diagnosis of pulmonary aspergillosis. Forty-four serum samples from patients with pulmonary aspergillosis (33 with pulmonary aspergilloma, 4 with allergic bronchopulmonary aspergillosis, 4 with invasive pulmonary aspergillosis, and 3 with aspergillus pyothorax) were used in this study. PCR detection of Aspergillus DNA in serum samples was successful in 39 patients. Galactomannan antigen was detected by sandwich enzyme-linked immunosorbent assay in 25 patients and by latex agglutination test in 13 patients. Detection of Aspergillus DNA in serum samples by nested PCR had the highest sensitivity of the three methods tested for the diagnosis of pulmonary aspergillosis.

* Corresponding author. Mailing address: Second Department of Internal Medicine, Nagasaki University School of Medicine, Sakamoto 1-7-1, Nagasaki 852-8501, Japan. Phone: 81-95-849-7271. Fax: 81-95-849-7285. E-mail: sk1227{at}net.nagasaki-u.ac.jp.

Journal of Clinical Microbiology, January 1999, p. 218-220, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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