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Journal of Clinical Microbiology, January 1999, p. 221-224, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Dissemination of High-Level Penicillin-, Extended-Spectrum Cephalosporin-, and Erythromycin-Resistant Streptococcus pneumoniae Clones in Taiwan

Po-Ren Hsueh,1,2 Lee-Jene Teng,3 Li-Na Lee,1,2 Pan-Chyr Yang,2 Shen-Wu Ho,3 and Kwen-Tay Luh1,2,*

Departments of Laboratory Medicine1 and Internal Medicine,2 National Taiwan University Hospital, and School of Medical Technology, National Taiwan University College of Medicine,3 Taipei, Taiwan

Received 17 August 1998/Returned for modification 16 September 1998/Accepted 15 October 1998

Sixty-seven clinical isolates of Streptococcus pneumoniae (40 of serotype 23F, 19 of serotype 19F, and 8 of serotype 6B) with decreased susceptibilities to penicillin and erythromycin were characterized by antimicrobial susceptibility patterns; DNA restriction endonuclease cleavage profiles of the penicillin-binding protein genes pbp1a, pbp2b, and pbp2x; random amplified polymorphic DNA (RAPD) patterns generated by arbitrarily primed PCR; and chromosomal macrorestriction profiles based on pulsed-field gel electrophoresis. A total of 22 clones (identical or closely related pulsotypes and identical RAPD patterns) were identified; 14 clones of 23F, 6 of 19F, and 2 of 6B. Three 23F clones (26 isolates) and one 19F clone (9 isolates) expressed high-level resistance to penicillin, cefotaxime, and erythromycin (MICs >=  256 µg/ml). These data strongly suggest that multiple high-level penicillin-, extended-spectrum cephalosporin-, and macrolide-resistant clones of S. pneumoniae have been disseminated in Taiwan.

* Corresponding author. Mailing address: Department of Laboratory Medicine, National Taiwan University Hospital, No. 7 Chung-Shan South Rd., Taipei, Taiwan. Phone: 886-2-23562149. Fax: 886-2-23224263. E-mail: luhkt{at}ha.mc.ntu.edu.tw.

Journal of Clinical Microbiology, January 1999, p. 221-224, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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