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Journal of Clinical Microbiology, January 1999, p. 251-254, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis of Legionella pneumophila Serogroup 1 Isolates by Pulsed-Field Gel Electrophoresis

Thomas J. Marrie,1,2,* Shaun Tyler,3 Gregory Bezanson,2 Charles Dendy,3 and Wendy Johnson3

Departments of Medicine1 and Microbiology,2 Dalhousie University and Queen Elizabeth II Health Sciences Centre, and Laboratory Centre for Disease Control, Department of Health,3 Ottawa, Ontario, Canada

Received 20 February 1998/Returned for modification 23 June 1998/Accepted 2 October 1998

At a hospital in Halifax, Nova Scotia, Canada, three strains of Legionella pneumophila were detectable based on plasmid content, while the isolates collected at another hospital in Halifax had no plasmids. Genomic DNA was digested with BssHII, SalI, and SpeI and subjected to pulsed-field gel electrophoresis (PFGE). We found no relationship between plasmid profile and PFGE pattern.

* Corresponding author. Mailing address: Department of Medicine, Queen Elizabeth II Health Sciences Centre, VG Site, Room 5014 ACC, 1278 Tower Rd., Halifax, Nova Scotia B3H 2Y9 Canada. Phone: 902 473-5553. Fax: 902 473-7394. E-mail: tmarrie{at}is.dal.ca.

Journal of Clinical Microbiology, January 1999, p. 251-254, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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