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Journal of Clinical Microbiology, January 1999, p. 261-262, Vol. 37, No. 1
0095-1137/99/$00.00+0

Optimal Activation of Isopsoralen To Prevent Amplicon Carryover

Gary A. Fahle,* Vee J. Gill, and Steven H. Fischer

Microbiology Service, Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508

Received 29 June 1998/Returned for modification 20 August 1998/Accepted 2 October 1998

We compared the efficiencies of activation of the photochemical isopsoralen compound 10 and its resulting amplicon neutralizations under conditions with a UV transilluminator box at room temperature (RT) and a HRI-300 UV photothermal reaction chamber at RT and at 5°C. Our data suggest that use of the HRI-300 reaction chamber at 5°C results in a statistically significantly higher degree of amplicon neutralization.

* Corresponding author. Mailing address: Microbiology Service, Building 10, Room 2C-385, National Institutes of Health, Bethesda, MD 20892-1508. Phone: (301) 496-4433. Fax: (301) 402-1886. E-mail: gfahle{at}nih.gov.

Journal of Clinical Microbiology, January 1999, p. 261-262, Vol. 37, No. 1
0095-1137/99/$00.00+0


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