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Journal of Clinical Microbiology, January 1999, p. 263-265, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Serotyping of Streptococcus pneumoniae by Coagglutination with 12 Pooled Antisera

M. K. Lalitha,1,* Kurien Thomas,2 R. Satish Kumar,1 Mark C. Steinhoff,3 and the IBIS Study Groupdagger

Department of Clinical Microbiology1 and Department of Medicine Unit II,2 Christian Medical College & Hospital, Vellore, India, and Department of International Health, School of Hygiene & Public Health, Johns Hopkins University, Baltimore, Maryland 212053

Received 18 May 1998/Returned for modification 18 August 1998/Accepted 13 October 1998

We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strains of S. pneumoniae studied, 135 (90%) belonged to the SGTs represented in the 23-valent pneumococcal vaccine; of these, 130 (96.3%) were identified as the same SGTs by both typing methods. The remaining five strains showed cross-reactivity with more than two pools by the chessboard method, but could be assigned to a single SGT by the Quellung test. The 96.3% concordance of the chessboard method suggests it can be adopted for determination of the SGTs of S. pneumoniae in laboratories.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore - 632004, India. Phone: 91-416-22102, ext. 2033. Fax: 91-416-32103/32035. E-mail: mkl{at}micro.cmc.ernet.in.

dagger Members of the IBIS (Invasive Bacterial Infection Surveillance) Study Group (India) are N. K. Arora (New Delhi), Bimal Das (New Delhi), Dipty Jain (Nagpur), Vandana Agarwal (Nagpur), Shally Awasti (Lucknow), Amita Jain (Lucknow), N. Deivanayagam (Chennai), R. Manjula (Chennai), P. Raghupathy (Vellore), Thomas Cherian (Vellore), and Reba Kanungo (Pondicherry).

Journal of Clinical Microbiology, January 1999, p. 263-265, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.