This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lalitha, M. K.
Right arrow Articles by the IBIS Study Group
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lalitha, M. K.
Right arrow Articles by the IBIS Study Group,

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 1999, p. 263-265, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Serotyping of Streptococcus pneumoniae by Coagglutination with 12 Pooled Antisera

M. K. Lalitha,1,* Kurien Thomas,2 R. Satish Kumar,1 Mark C. Steinhoff,3 and the IBIS Study Groupdagger

Department of Clinical Microbiology1 and Department of Medicine Unit II,2 Christian Medical College & Hospital, Vellore, India, and Department of International Health, School of Hygiene & Public Health, Johns Hopkins University, Baltimore, Maryland 212053

Received 18 May 1998/Returned for modification 18 August 1998/Accepted 13 October 1998

We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strains of S. pneumoniae studied, 135 (90%) belonged to the SGTs represented in the 23-valent pneumococcal vaccine; of these, 130 (96.3%) were identified as the same SGTs by both typing methods. The remaining five strains showed cross-reactivity with more than two pools by the chessboard method, but could be assigned to a single SGT by the Quellung test. The 96.3% concordance of the chessboard method suggests it can be adopted for determination of the SGTs of S. pneumoniae in laboratories.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore - 632004, India. Phone: 91-416-22102, ext. 2033. Fax: 91-416-32103/32035. E-mail: mkl{at}micro.cmc.ernet.in.

dagger Members of the IBIS (Invasive Bacterial Infection Surveillance) Study Group (India) are N. K. Arora (New Delhi), Bimal Das (New Delhi), Dipty Jain (Nagpur), Vandana Agarwal (Nagpur), Shally Awasti (Lucknow), Amita Jain (Lucknow), N. Deivanayagam (Chennai), R. Manjula (Chennai), P. Raghupathy (Vellore), Thomas Cherian (Vellore), and Reba Kanungo (Pondicherry).

Journal of Clinical Microbiology, January 1999, p. 263-265, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Findlow, H., Laher, G., Balmer, P., Broughton, C., Carrol, E. D., Borrow, R. (2009). Competitive Inhibition Flow Analysis Assay for the Non-Culture-Based Detection and Serotyping of Pneumococcal Capsular Polysaccharide. CVI 16: 222-229 [Abstract] [Full Text]  
  • HERVA, E., GRANAT, S., MIA, Z., OLLGREN, J., PIIRAINEN, L., MAKELA, P. H. (2006). Field evaluation of the chessboard modification for serotyping of streptococcus pneumoniae in a small laboratory in bangladesh.. Am J Trop Med Hyg 74: 863-867 [Abstract] [Full Text]  
  • Batt, S. L., Charalambous, B. M., McHugh, T. D., Martin, S., Gillespie, S. H. (2005). Novel PCR-Restriction Fragment Length Polymorphism Method for Determining Serotypes or Serogroups of Streptococcus pneumoniae Isolates. J. Clin. Microbiol. 43: 2656-2661 [Abstract] [Full Text]  
  • Shutt, C. K., Samore, M., Carroll, K. C. (2004). Comparison of the Denka Seiken Slide Agglutination Method to the Quellung Test for Serogrouping of Streptococcus pneumoniae Isolates. J. Clin. Microbiol. 42: 1274-1276 [Abstract] [Full Text]  
  • Kong, F., Gilbert, G. L. (2003). Using cpsA-cpsB sequence polymorphisms and serotype-/group-specific PCR to predict 51 Streptococcus pneumoniae capsular serotypes. J Med Microbiol 52: 1047-1058 [Abstract] [Full Text]  
  • Brito, D. A., Ramirez, M., de Lencastre, H. (2003). Serotyping Streptococcus pneumoniae by Multiplex PCR. J. Clin. Microbiol. 41: 2378-2384 [Abstract] [Full Text]  
  • Coles, C. L., Rahmathullah, L., Kanungo, R., Thulasiraj, R. D., Katz, J., Santhosham, M., Tielsch, J. M. (2001). Vitamin A Supplementation at Birth Delays Pneumococcal Colonization in South Indian Infants. J. Nutr. 131: 255-261 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»