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Journal of Clinical Microbiology, January 1999, p. 81-89, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Molecular Epidemiology of Outbreaks of Gastroenteritis Associated
with Small Round-Structured Viruses in East Anglia, United Kingdom,
During the 1996-1997 Season
Alison J.
Maguire,1
Jon
Green,2
David W. G.
Brown,2
Ulrich
Desselberger,1 and
James J.
Gray1,*
Clinical Microbiology and Public Health
Laboratory, Addenbrooke's Hospital, Cambridge CB2
2QW,1 and
Enteric and Respiratory
Virus Laboratory, Virus Reference Division, Central Public Health
Laboratory, Colindale, London NW9 5HT,2 United
Kingdom
Received 8 June 1998/Returned for modification 3 September
1998/Accepted 7 October 1998
During the winter season from November 1996 to May 1997, 550 fecal
specimens were submitted from 94 outbreaks of gastroenteritis occurring
in East Anglia, United Kingdom. These specimens were tested for the
presence of small round-structured viruses (SRSVs) by electron
microscopy, reverse transcriptase PCR, or both methods. SRSVs were
shown to be associated with 64 of 94 (68%) of these outbreaks, of
which 16 (25%) outbreaks occurred at a single location (Southend)
within the region. Twenty-four specimens from 13 of the 16 SRSV-positive outbreaks occurring in Southend were available for
genomic analysis, in which divergence within the RNA polymerase region
of the SRSV genome was investigated. A further 27 specimens from 17 other SRSV-associated outbreaks, occurring at different locations
within East Anglia but at the same time as those at Southend, were also
studied. Fifty of the total of 51 (98%) specimens studied were shown
to belong to genogroup II, and within this genogroup, 49 of 50 (98%) specimens were shown to be Grimsby-like viruses, with only one
Mexico-like strain. Furthermore, phylogenetic analysis of the
Grimsby-like viruses indicated clusterings according to the
geographical location of the outbreak. One specimen contained a virus
belonging to genogroup I, and this had the greatest sequence identity (83%) with Southampton virus.
*
Corresponding author. Mailing address: Clinical
Microbiology and Public Health Laboratory, Level 6, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW, United Kingdom.
Phone: 44-1223-257028. Fax: 44-1223-242775. E-mail:
jg2{at}mole.bio.cam.ac.uk.
Journal of Clinical Microbiology, January 1999, p. 81-89, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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