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Journal of Clinical Microbiology, January 1999, p. 99-102, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Identification of Clinically Relevant Nocardia Species to Genus Level by 16S rRNA Gene PCR

Frederic J. Laurent,1,2,* Frederique Provost,1 and Patrick Boiron1

Institut Pasteur, Unité de Mycologie, Centre National de Référence des Mycoses Humaines, des Antifongiques et des Actinomycètes, 75724 Paris Cedex 15,1 and Laboratoire de Bactériologie, C.H.U. Lyon Sud, 69495 Pierre Bénite Cedex,2 France

Received 15 May 1998/Returned for modification 8 July 1998/Accepted 25 September 1998

Two regions of the gene coding for 16S rRNA in Nocardia species were selected as genus-specific primer sequences for a PCR assay. The PCR protocol was tested with 60 strains of clinically relevant Nocardia isolates and type strains. It gave positive results for all strains tested. Conversely, the PCR assay was negative for all tested species belonging to the most closely related genera, including Dietzia, Gordona, Mycobacterium, Rhodococcus, Streptomyces, and Tsukamurella. Besides, unlike the latter group of isolates, all Nocardia strains exhibited one MlnI recognition site but no SacI restriction site. This assay offers a specific and rapid alternative to chemotaxonomic methods for the identification of Nocardia spp. isolated from pathogenic samples.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, C.H.U. Lyon Sud, Chemin du Grand Revoyet, 69495 Pierre Bénite Cedex, France. Phone: (33) (0) 4 78 86 12 32. Fax: (33) (0) 4 78 86 32 80. E-mail: laurent{at}lyon-sud.univ-lyon1.fr.

Journal of Clinical Microbiology, January 1999, p. 99-102, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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