Determination of Chlamydia trachomatis Prevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens

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Journal of Clinical Microbiology, October 1999, p. 3092-3096, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Determination of Chlamydia trachomatis Prevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens

Servaas A. Morré,¹ Irene G. M. Van Valkengoed,² Robert M. Moes,¹ A. Joan P. Boeke,² Chris J. L. M. Meijer,¹ and Adriaan J. C. Van den Brule¹^,^*

Department of Pathology, Section of Molecular Pathology, University Hospital Vrije Universiteit,¹ and Institute for Research in Extramural Medicine, Vrije Universiteit,² Amsterdam, The Netherlands

Received 7 May 1999/Returned for modification 8 June 1999/Accepted 29 June 1999

This study determined the performances of the LCx (Abbott) and COBAS Amplicor (Roche) tests with urine specimens for the detectionof Chlamydia trachomatis in an asymptomatic screening population.Randomly selected women and men (age range, 15 to 40 years) registeredin 20 general practices in Amsterdam, The Netherlands, were invitedto participate in this study. Urine specimens (n = 2,906; 1,138specimens from men and 1,717 specimens from women) were testedfor C. trachomatis by the COBAS Amplicor (Roche) and LCx (Abbott)tests. Samples which were positive by only one assay were subjectedto discrepant analyses by a third assay (in-house plasmid PCR).By the LCx assay C. trachomatis DNA was detected in urine specimensfrom 46 of 1,717 women and 29 of 1,138 men, while the COBAS Amplicordetected C. trachomatis DNA in 52 and 35 specimens, respectively.When comparing the LCx and COBAS Amplicor tests, 32 test results(20 for women and 12 for men) were discrepant. After discrepantanalyses the following sensitivities, specificities, and positivepredictive values were found for the LCx and COBAS Amplicor tests:78.6 versus 98.8%, 99.7 versus 99.9%, and 88.0 versus 95.4%, respectively.No prominent differences were found between men and women withregard to the test performances. After discrepant analyses theoverall prevalences of C. trachomatis in women and men were 3.0and 2.8%, respectively. For both women and men the prevalencein the younger age groups was higher than that in the older agegroups. In conclusion, the COBAS Amplicor tests shows better diagnosticcharacteristics than the LCx assay for the detection of C. trachomatisin urine specimens from an asymptomatic screening population.In this asymptomatic population the overall prevalence of C. trachomatiswas 2.9%.

^* Corresponding author. Mailing address: Department of Pathology, Section of Molecular Pathology, University Hospital VrijeUniversiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.Phone: 31-20-4440503 or 31-20-4444023. Fax: 31-20-4442964. E-mail:vandenbrule{at}azvu.nl.

Journal of Clinical Microbiology, October 1999, p. 3092-3096, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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