This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pfyffer, G. E.
Right arrow Articles by Siddiqi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pfyffer, G. E.
Right arrow Articles by Siddiqi, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 1999, p. 3179-3186, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multicenter Laboratory Validation of Susceptibility Testing of Mycobacterium tuberculosis against Classical Second-Line and Newer Antimicrobial Drugs by Using the Radiometric BACTEC 460 Technique and the Proportion Method with Solid Media

Gaby E. Pfyffer,1,* Donald A. Bonato,2 Adeleh Ebrahimzadeh,3 Wendy Gross,2 Jacqueline Hotaling,4 John Kornblum,3 Adalbert Laszlo,5 Glenn Roberts,6 Max Salfinger,4 Franziska Wittwer,1 and Salman Siddiqi7

Swiss National Center for Mycobacteria, Department of Medical Microbiology, University of Zurich, Zurich, Switzerland1; Veterans Affairs Medical Center, West Haven, Connecticut2; New York City Department of Health, New York,3 and Wadsworth Center, New York State Department of Health, Albany,4 New York; Laboratory Center for Disease Control, Ottawa, Canada5; Mayo Clinic, Rochester, Minnesota6; and Becton Dickinson Microbiology Systems, Sparks, Maryland7

Received 10 May 1999/Returned for modification 11 July 1999/Accepted 14 July 1999

In a large multicenter study involving six major study sites in the United States, Canada, and Europe, the susceptibilities of 272 Mycobacterium tuberculosis strains to classical second-line antituberculosis (anti-TB) drugs (capreomycin, cycloserine, ethionamide, and kanamycin) and newer compounds (amikacin, clofazimine, ofloxacin, and rifabutin) were determined by the radiometric BACTEC 460 procedure and the conventional proportion method on Middlebrook 7H10 agar. Previously established critical concentrations for classical second-line anti-TB drugs were compared with several concentrations in liquid medium to establish equivalence. MICs of newer compounds determined in liquid medium were either the same or up to four times lower than those determined in agar medium. After establishing critical concentrations (breakpoints) in the extended testing of clinical isolates, we obtained an excellent overall correlation between the two systems, with no errors with amikacin, kanamycin, and ofloxacin and very few major or very major errors with the other drugs; however, for cycloserine, no breakpoint concentration could be recommended due to repeatedly inconsistent results by both methods. Based on these data we conclude that the BACTEC 460 procedure is a simple and rapid method requiring 4 to 8 days on average to generate accurate antimicrobial susceptibility testing (AST) results for eight anti-TB drugs other than those considered primary ones. These data not only fill a major gap of knowledge regarding the critical test concentrations of secondary anti-TB drugs but also provide a baseline for future evaluations of M. tuberculosis AST with the more recently developed, nonradiometric broth-based culture systems.

* Corresponding author. Mailing address: Swiss National Center for Mycobacteria, Department of Medical Microbiology, University of Zurich, Gloriastrasse 30, 8028 Zurich, Switzerland. Phone: 41 1 634 27 86. Fax: 41 1 634 49 18. E-mail: pfyffer{at}immv.unizh.ch.

Journal of Clinical Microbiology, October 1999, p. 3179-3186, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lin, S.-Y. G., Desmond, E., Bonato, D., Gross, W., Siddiqi, S. (2009). Multicenter Evaluation of Bactec MGIT 960 System for Second-Line Drug Susceptibility Testing of Mycobacterium tuberculosis Complex. J. Clin. Microbiol. 47: 3630-3634 [Abstract] [Full Text]  
  • Feuerriegel, S., Cox, H. S., Zarkua, N., Karimovich, H. A., Braker, K., Rusch-Gerdes, S., Niemann, S. (2009). Sequence Analyses of Just Four Genes To Detect Extensively Drug-Resistant Mycobacterium tuberculosis Strains in Multidrug-Resistant Tuberculosis Patients Undergoing Treatment. Antimicrob. Agents Chemother. 53: 3353-3356 [Abstract] [Full Text]  
  • Springer, B., Lucke, K., Calligaris-Maibach, R., Ritter, C., Bottger, E. C. (2009). Quantitative Drug Susceptibility Testing of Mycobacterium tuberculosis by Use of MGIT 960 and EpiCenter Instrumentation. J. Clin. Microbiol. 47: 1773-1780 [Abstract] [Full Text]  
  • Martin, A., von Groll, A., Fissette, K., Palomino, J. C., Varaine, F., Portaels, F. (2008). Rapid Detection of Mycobacterium tuberculosis Resistance to Second-Line Drugs by Use of the Manual Mycobacterium Growth Indicator Tube System. J. Clin. Microbiol. 46: 3952-3956 [Abstract] [Full Text]  
  • Rusch-Gerdes, S., Pfyffer, G. E., Casal, M., Chadwick, M., Siddiqi, S. (2006). Multicenter Laboratory Validation of the BACTEC MGIT 960 Technique for Testing Susceptibilities of Mycobacterium tuberculosis to Classical Second-Line Drugs and Newer Antimicrobials.. J. Clin. Microbiol. 44: 688-692 [Abstract] [Full Text]  
  • Kruuner, A., Yates, M. D., Drobniewski, F. A. (2006). Evaluation of MGIT 960-Based Antimicrobial Testing and Determination of Critical Concentrations of First- and Second-Line Antimicrobial Drugs with Drug-Resistant Clinical Strains of Mycobacterium tuberculosis.. J. Clin. Microbiol. 44: 811-818 [Abstract] [Full Text]  
  • Takakura, S., Tsuchiya, S., Fujihara, N., Kudo, T., Iinuma, Y., Mitarai, S., Ichiyama, S., Yasukawa, K., Ishiguro, T. (2005). Isothermal RNA Sequence Amplification Method for Rapid Antituberculosis Drug Susceptibility Testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 43: 2489-2491 [Abstract] [Full Text]  
  • Martin, A., Palomino, J. C., Portaels, F. (2005). Rapid Detection of Ofloxacin Resistance in Mycobacterium tuberculosis by Two Low-Cost Colorimetric Methods: Resazurin and Nitrate Reductase Assays. J. Clin. Microbiol. 43: 1612-1616 [Abstract] [Full Text]  
  • Kim, S. J. (2005). Drug-susceptibility testing in tuberculosis: methods and reliability of results. Eur Respir J 25: 564-569 [Abstract] [Full Text]  
  • Sanders, C. A., Nieda, R. R., Desmond, E. P. (2004). Validation of the Use of Middlebrook 7H10 Agar, BACTEC MGIT 960, and BACTEC 460 12B Media for Testing the Susceptibility of Mycobacterium tuberculosis to Levofloxacin. J. Clin. Microbiol. 42: 5225-5228 [Abstract] [Full Text]  
  • Di Perri, G., Bonora, S. (2004). Which agents should we use for the treatment of multidrug-resistant Mycobacterium tuberculosis?. J Antimicrob Chemother 54: 593-602 [Abstract] [Full Text]  
  • Huang, T.-S., Lee, S. S.-J., Tu, H.-Z., Huang, W.-K., Chen, Y.-S., Huang, C.-K., Wann, S.-R., Lin, H.-H., Liu, Y.-C. (2004). Use of MGIT 960 for rapid quantitative measurement of the susceptibility of Mycobacterium tuberculosis complex to ciprofloxacin and ethionamide. J Antimicrob Chemother 53: 600-603 [Abstract] [Full Text]  
  • Martin, A., Camacho, M., Portaels, F., Palomino, J. C. (2003). Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method. Antimicrob. Agents Chemother. 47: 3616-3619 [Abstract] [Full Text]  
  • Kruuner, A., Jureen, P., Levina, K., Ghebremichael, S., Hoffner, S. (2003). Discordant Resistance to Kanamycin and Amikacin in Drug-Resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 47: 2971-2973 [Abstract] [Full Text]  
  • Bulatovic, V. M., Wengenack, N. L., Uhl, J. R., Hall, L., Roberts, G. D., Cockerill, F. R. III, Rusnak, F. (2002). Oxidative Stress Increases Susceptibility of Mycobacterium tuberculosis to Isoniazid. Antimicrob. Agents Chemother. 46: 2765-2771 [Abstract] [Full Text]  
  • Bastian, I., Rigouts, L., Palomino, J. C., Portaels, F. (2001). Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method. Antimicrob. Agents Chemother. 45: 1934-1936 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»