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Journal of Clinical Microbiology, October 1999, p. 3276-3280, Vol. 37, No. 10
0095-1137/99/$04.00+0

Ciprofloxacin Resistance in Campylobacter jejuni Isolates: Detection of gyrA Resistance Mutations by Mismatch Amplification Mutation Assay PCR and DNA Sequence Analysis

Gerald Zirnstein,* Yu Li, Bala Swaminathan, and Frederick Angulo

National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia

Received 19 May 1999/Returned for modification 7 June 1999/Accepted 9 July 1999

The gyrA quinolone resistance determining region was sequenced from 13 ciprofloxacin-resistant and 20 ciprofloxacin-susceptible Campylobacter jejuni isolates. All isolates resistant to ciprofloxacin had Thr-86-to-Ile mutations, a mutation frequently associated with the acquisition of resistance to fluoroquinolones. A mismatch amplification mutation assay (MAMA) PCR protocol was developed that detects this gyrA mutation in quinolone-resistant isolates. The MAMA PCR provides a means for routine detection of the gyrA mutation without the need for sequencing the gyrA gene.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Mail Stop C03, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 321-6111, ext. 2537. Fax: (404) 728-7750. E-mail: gaz3{at}cdc.gov.

Journal of Clinical Microbiology, October 1999, p. 3276-3280, Vol. 37, No. 10
0095-1137/99/$04.00+0


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