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Journal of Clinical Microbiology, October 1999, p. 3350-3352, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Quantification of Human Cytomegalovirus DNA in Amniotic Fluid of Mothers of Congenitally Infected Fetuses

Maria Grazia Revello,* Maurizio Zavattoni, Milena Furione, Fausto Baldanti, and Giuseppe Gerna

Servizio di Virologia, IRCCS Policlinico San Matteo, 27100 Pavia, Italy

Received 10 May 1999/Returned for modification 24 June 1999/Accepted 22 July 1999

A quantitative PCR assay was used to quantitate human cytomegalovirus DNA in amniotic fluid of mothers of 21 fetuses with congenital infection. Seven fetuses presented ultrasound abnormalities or were born with symptoms, whereas 14 fetuses were subclinically infected. Although the median DNA level was higher in symptomatic fetuses, the difference was not statistically significant (P = 0.09).

* Corresponding author. Mailing address: Servizio di Virologia, IRCCS Policlinico San Matteo, 27100 Pavia, Italy. Phone: 39-0382-502634. Fax: 39-0382-502599. E-mail: virology{at}ipv36.unipv.it.

Journal of Clinical Microbiology, October 1999, p. 3350-3352, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.