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Journal of Clinical Microbiology, October 1999, p. 3366-3368, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Identification of a Nocardiopsis dassonvillei Blood Isolate

Frédéric Beau,1 Claude Bollet,1 Thierry Coton,2 Eric Garnotel,3 and Michel Drancourt1,*

Laboratoire de Bactériologie, Assistance Publique---Hôpitaux de Marseille,1 and Service de Gastro-entérologie2 and Laboratoire de Microbiologie,3 Hôpital d'Instruction des Armées Alphonse Laveran, Marseille, France

Received 28 April 1999/Returned for modification 30 June 1999/Accepted 22 July 1999

Nocardiopsis dassonvillei is an environmental aerobic actinomycete seldom isolated in cutaneous and pulmonary infections. We herein report the first N. dassonvillei blood isolate in a patient hospitalized for cholangitis. Although morphological characteristics and biochemical tests allowed a presumptive identification of this isolate, cell wall fatty acid chromatographic analysis confirmed identification at the genus level, and 16S rRNA gene sequencing achieved definite identification. This study illustrates the usefulness of 16S rRNA gene sequencing as a routine method for the identification of actinomycetes.

* Corresponding author. Present address: Unité des Rickettsies, CNRS UPRESA 6002, Faculté de Médecine, Université de la Méditerranée, 27 Blvd. Jean Moulin, 13385 Marseille Cedex 05, France. Phone: 33 04 91 38 55 17. Fax: 33 04 91 83 03 90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, October 1999, p. 3366-3368, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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