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Journal of Clinical Microbiology, October 1999, p. 3370-3373, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Specific PCR Identification and Differentiation of the Thermophilic Campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis

Christian Fermér1,* and Eva Olsson Engvall2

National Food Administration, Biology Division, SE-751 26 Uppsala,1 and National Veterinary Institute, Swedish Zoonosis Center, S-750 07 Uppsala,2 Sweden

Received 1 February 1999/Returned for modification 23 March 1999/Accepted 5 July 1999

A sensitive PCR assay that detects the thermophilic campylobacters C. jejuni, C. coli, C. lari, and C. upsaliensis is reported. Furthermore, by digestion of the PCR products with two restriction enzymes, species differentiation was demonstrated. Thus, the present method has the potential to be used for both detection and identification of thermophilic Campylobacter species.

* Corresponding author. Mailing address: National Food Administration, Biology Division, P.O. Box 622, SE-751 26 Uppsala, Sweden. Phone: 46 18 17 14 20. Fax: 46 18 17 14 94. E-mail: chfe{at}slv.se.

Journal of Clinical Microbiology, October 1999, p. 3370-3373, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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