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Journal of Clinical Microbiology, October 1999, p. 3392-3394, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Adenovirus Strains of Subgenus D Associated with Nosocomial Infection as New Etiological Agents of Epidemic Keratoconjunctivitis in Japan

Satoshi Takeuchi,1,* Norihiko Itoh,1 Eiichi Uchio,1 Keico Tanaka,1 Noriko Kitamura,1 Hikaru Kanai,1 Kazumi Isobe,1 Koki Aoki,2 and Shigeaki Ohno1

Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004,1 and Aoki Eye Clinic, Shiroishi-ku, Sapporo 003-0027,2 Japan

Received 5 March 1999/Returned for modification 25 May 1999/Accepted 5 July 1999

Adenovirus strains of a new type were isolated from patients with epidemic keratoconjunctivitis. They were not completely neutralized by any antiserum against adenovirus prototypes. PCR followed by restriction endonuclease analysis demonstrated that they were type 8. PCR followed by sequencing revealed a high homology rate between them and type 9.

* Corresponding author. Mailing address: Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan. Phone: 81-45-787-2683. Fax: 81-45-781-9755. E-mail: takeuchi{at}med.yokohama-cu.ac.jp.

Journal of Clinical Microbiology, October 1999, p. 3392-3394, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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