Previous Article | Next Article 
Journal of Clinical Microbiology, November 1999, p. 3452-3457, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Emergence and Spread in French Hospitals of
Methicillin-Resistant Staphylococcus aureus with Increasing
Susceptibility to Gentamicin and Other Antibiotics
Hervé
Lelièvre,1
Gerard
Lina,1
Mark E.
Jones,2,
Claude
Olive,3
Françoise
Forey,1
Micheline
Roussel-Delvallez,4
Marie-Hélène
Nicolas-Chanoine,5
Cécile M.
Bébéar,6
Vincent
Jarlier,7
Antoine
Andremont,8
François
Vandenesch,1 and
Jerome
Etienne1,*
Centre National de Référence des
Toxémies à Staphylocoques, EA 1655, Faculté de
Médecine, 69372 Lyon Cedex 08,1
Laboratoire de Microbiologie, Hôpital Pierre Zobda
Quitman, 97261 Fort de France,3
Laboratoire de Bactériologie, Hôpital Calmette,
59037 Lille Cedex,4 Laboratoire de
Microbiologie, Hôpital Ambroise Paré, 92100 Boulogne,5 Laboratoire de
Bactériologie, Hôpital Pellegrin, 33076 Bordeaux
Cedex,6 Laboratoire de
Bactériologie et Hygiène, Groupe Hospitalier Pitié
Salpétrière, 75651 Paris Cedex 13,7
and Laboratoire de Bactériologie, CHU Bichat-Claude
Bernard, 75877 Paris Cedex 18,8 France, and
University Hospital of Utrecht, 3584CX Utrecht, The
Netherlands2
Received 7 May 1999/Returned for modification 6 July 1999/Accepted 29 July 1999
Oxacillin (methicillin) resistance in methicillin-resistant
Staphylococcus aureus (MRSA) is associated with an
increased incidence of resistance to other antibiotics, which has
increased since it was first reported in 1969. In 1992 a new
phenotype of MRSA arose in France; this was characterized by a
heterogeneous expression of resistance to oxacillin and susceptibility
to various antibiotics, including gentamicin but also tetracycline,
minocycline, lincomycin, pristinamycin, co-trimoxazole, rifampin, and
fusidic acid. In French hospitals a longitudinal nationwide
surveillance of antibiotic resistance in S. aureus has
allowed for the detection of changes in antibiotic susceptibility
profiles. Seven French clinical laboratories (six from the mainland and
one from the West Indies) reported the results of susceptibility
testing of 57,347 S. aureus strains isolated in their
institutes between 1992 and 1998. Over a 7-year period the incidence of
isolation of gentamicin-susceptible MRSA (GS-MRSA) strains has steadily
increased to represent, in 1998, 46.8 to 94.4% of the MRSA strains,
irrespective of the overall incidence of MRSA. Two predominant types
recognized by pulsed-field gel electrophoresis (PFGE) accounted for the
majority of the GS-MRSA in different mainland hospitals, both differing
from the predominant type observed in the French West Indies. Some
GS-MRSA and gentamicin-resistant MRSA (GR-MRSA) strains had closely
related PFGE profiles, and hybridization studies confirmed the lack in
GS-MRSA of the aac6'-aph2" gene, which confers resistance
to all aminoglycosides, with conservation of the ant4'
gene, which confers resistance to kanamycin, tobramycin, and amikacin.
Thus, it is likely that certain GS-MRSA strains could have emerged from
GR-MRSA strains by excision or deletion of the aac6'-aph2" gene.
*
Corresponding author. Mailing address: Faculté de
Médecine, Laboratoire de Bactériologie, Rue Guillaume
Paradin, 69372 Lyon Cedex 08, France. Phone: 33 (0) 478 77 86 57. Fax:
33 (0) 478 77 86 58. E-mail: jetienne{at}univ-lyon1.fr.
Present address: MRL Pharmaceutical Services, 3554XD, Utrecht, The Netherlands.
Journal of Clinical Microbiology, November 1999, p. 3452-3457, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Dauwalder, O., Lina, G., Durand, G., Bes, M., Meugnier, H., Jarlier, V., Coignard, B., Vandenesch, F., Etienne, J., Laurent, F.
(2008). Epidemiology of Invasive Methicillin-Resistant Staphylococcus aureus Clones Collected in France in 2006 and 2007. J. Clin. Microbiol.
46: 3454-3458
[Abstract]
[Full Text]
-
Kishii, K., Ito, T., Watanabe, S., Okuzumi, K., Hiramatsu, K.
(2008). Recurrence of heterogeneous methicillin-resistant Staphylococcus aureus (MRSA) among the MRSA clinical isolates in a Japanese university hospital. J Antimicrob Chemother
62: 324-328
[Abstract]
[Full Text]
-
Donnio, P.-Y., Fevrier, F., Bifani, P., Dehem, M., Kervegant, C., Wilhelm, N., Gautier-Lerestif, A.-L., Lafforgue, N., Cormier, M., the MR-MSSA Study Group of the College de Bacterio, , Le Coustumier, A.
(2007). Molecular and Epidemiological Evidence for Spread of Multiresistant Methicillin-Susceptible Staphylococcus aureus Strains in Hospitals. Antimicrob. Agents Chemother.
51: 4342-4350
[Abstract]
[Full Text]
-
Daskalaki, M., Otero, J. R., Sanz, F., Chaves, F.
(2007). Bacteremia Due to Clonally Derived Methicillin-Resistant, Gentamicin-Susceptible Isolates and Methicillin-Susceptible, Gentamicin-Resistant Isolates of Staphylococcus aureus. J. Clin. Microbiol.
45: 3446-3448
[Abstract]
[Full Text]
-
Amorim, M. L., Faria, N. A., Oliveira, D. C., Vasconcelos, C., Cabeda, J. C., Mendes, A. C., Calado, E., Castro, A. P., Ramos, M. H., Amorim, J. M., de Lencastre, H.
(2007). Changes in the Clonal Nature and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Isolates Associated with Spread of the EMRSA-15 Clone in a Tertiary Care Portuguese Hospital. J. Clin. Microbiol.
45: 2881-2888
[Abstract]
[Full Text]
-
Hallin, M., Denis, O., Deplano, A., De Mendonca, R., De Ryck, R., Rottiers, S., Struelens, M. J.
(2007). Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey. J Antimicrob Chemother
59: 465-472
[Abstract]
[Full Text]
-
Ferry, T., Bes, M., Dauwalder, O., Meugnier, H., Lina, G., Forey, F., Vandenesch, F., Etienne, J.
(2006). Toxin Gene Content of the Lyon Methicillin-Resistant Staphylococcus aureus Clone Compared with That of Other Pandemic Clones.. J. Clin. Microbiol.
44: 2642-2644
[Abstract]
[Full Text]
-
Layer, F., Ghebremedhin, B., Konig, W., Konig, B.
(2006). Heterogeneity of Methicillin-Susceptible Staphylococcus aureus Strains at a German University Hospital Implicates the Circulating-Strain Pool as a Potential Source of Emerging Methicillin-Resistant S. aureus Clones.. J. Clin. Microbiol.
44: 2179-2185
[Abstract]
[Full Text]
-
Vindel, A., Trincado, P., Gomez, E., Cabrera, R., Boquete, T., Sola, C., Valdezate, S., Saez-Nieto, J. A.
(2006). Prevalence and Evolution of Methicillin-Resistant Staphylococcus aureus in Spanish Hospitals between 1996 and 2002. J. Clin. Microbiol.
44: 266-270
[Abstract]
[Full Text]
-
van der Mee-Marquet, N., Domelier, A.-S., Girard, N., Quentin, R., the Bloodstream Infection Study Group of the Relai,
(2004). Epidemiology and Typing of Staphylococcus aureus Strains Isolated from Bloodstream Infections. J. Clin. Microbiol.
42: 5650-5657
[Abstract]
[Full Text]
-
Wannet, W. J. B., Spalburg, E., Heck, M. E. O. C., Pluister, G. N., Willems, R. J. L., de Neeling, A. J.
(2004). Widespread Dissemination in The Netherlands of the Epidemic Berlin Methicillin-Resistant Staphylococcus aureus Clone with Low-Level Resistance to Oxacillin. J. Clin. Microbiol.
42: 3077-3082
[Abstract]
[Full Text]
-
Oteo, J., Baquero, F., Vindel, A., Campos, J., on behalf of the Spanish members of The European A,
(2004). Antibiotic resistance in 3113 blood isolates of Staphylococcus aureus in 40 Spanish hospitals participating in the European Antimicrobial Resistance Surveillance System (2000-2002). J Antimicrob Chemother
53: 1033-1038
[Abstract]
[Full Text]
-
Donnio, P.-Y., Preney, L., Gautier-Lerestif, A.-L., Avril, J.-L., Lafforgue, N.
(2004). Changes in staphylococcal cassette chromosome type and antibiotic resistance profile in methicillin-resistant Staphylococcus aureus isolates from a French hospital over an 11 year period. J Antimicrob Chemother
53: 808-813
[Abstract]
[Full Text]
-
Strommenger, B., Kettlitz, C., Werner, G., Witte, W.
(2003). Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus. J. Clin. Microbiol.
41: 4089-4094
[Abstract]
[Full Text]
-
Thouverez, M., Muller, A., Hocquet, D., Talon, D., Bertrand, X.
(2003). Relationship between molecular epidemiology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) in a French teaching hospital. J Med Microbiol
52: 801-806
[Abstract]
[Full Text]
-
Kikuchi, K., Takahashi, N., Piao, C., Totsuka, K., Nishida, H., Uchiyama, T.
(2003). Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Strains Causing Neonatal Toxic Shock Syndrome-Like Exanthematous Disease in Neonatal and Perinatal Wards. J. Clin. Microbiol.
41: 3001-3006
[Abstract]
[Full Text]
-
Watson, J., Givney, R., Beard-Pegler, M., Rose, B., Merlino, J., Vickery, A., Gottlieb, T., Bradbury, R., Harbour, C.
(2003). Comparative Analysis of Multidrug-Resistant, Non-Multidrug-Resistant, and Archaic Methicillin-Resistant Staphylococcus aureus Isolates from Central Sydney, Australia. J. Clin. Microbiol.
41: 867-872
[Abstract]
[Full Text]
-
Heym, B., Le Moal, M., Armand-Lefevre, L., Nicolas-Chanoine, M.-H.
(2002). Multilocus sequence typing (MLST) shows that the 'Iberian' clone of methicillin-resistant Staphylococcusaureus has spread to France and acquired reduced susceptibility to teicoplanin. J Antimicrob Chemother
50: 323-329
[Abstract]
[Full Text]
-
Asseray, N., Caillon, J., Roux, N., Jacqueline, C., Bismuth, R., Kergueris, M. F., Potel, G., Bugnon, D.
(2002). Different Aminoglycoside-Resistant Phenotypes in a Rabbit Staphylococcus aureus Endocarditis Infection Model. Antimicrob. Agents Chemother.
46: 1591-1593
[Abstract]
[Full Text]
-
Donnio, P.-Y., Louvet, L., Preney, L., Nicolas, D., Avril, J.-L., Desbordes, L.
(2002). Nine-Year Surveillance of Methicillin-Resistant Staphylococcus aureus in a Hospital Suggests Instability of mecA DNA Region in an Epidemic Strain. J. Clin. Microbiol.
40: 1048-1052
[Abstract]
[Full Text]
-
Polyzou, A., Slavakis, A., Pournaras, S., Maniatis, A. N., Sofianou, D., Tsakris, A.
(2001). Predominance of a methicillin-resistant Staphylococcus aureus clone susceptible to erythromycin and several other non-{beta}-lactam antibiotics in a Greek hospital. J Antimicrob Chemother
48: 231-234
[Abstract]
[Full Text]
-
Blanc, D. S., Francioli, P., Le Coustumier, A., Gazagne, L., Lecaillon, E., Gueudet, P., Vandenesch, F., Etienne, J.
(2001). Reemergence of Gentamicin-Susceptible Strains of Methicillin-Resistant Staphylococcus aureus in France: a Phylogenetic Approach. J. Clin. Microbiol.
39: 2287-2290
[Abstract]
[Full Text]
-
Laurent, F., Lelievre, H., Cornu, M., Vandenesch, F., Carret, G., Etienne, J., Flandrois, J.-P.
(2001). Fitness and competitive growth advantage of new gentamicin-susceptible MRSA clones spreading in French hospitals. J Antimicrob Chemother
47: 277-283
[Abstract]
[Full Text]
-
Pournaras, S., Slavakis, A., Polyzou, A., Sofianou, D., Maniatis, A. N., Tsakris, A.
(2001). Nosocomial Spread of an Unusual Methicillin-Resistant Staphylococcus aureus Clone That Is Sensitive to All Non-{beta}-Lactam Antibiotics, Including Tobramycin. J. Clin. Microbiol.
39: 779-781
[Abstract]
[Full Text]
-
Carricajo, A., Fonsale, N., Vautrin, A. C., Aubert, G.
(2000). In vitro activity of {beta}-lactams against gentamicin-susceptible and gentamicin-resistant Staphylococcus aureus. J Antimicrob Chemother
46: 856-858
[Full Text]
-
Nimmo, G. R., Schooneveldt, J., O'Kane, G., McCall, B., Vickery, A.
(2000). Community Acquisition of Gentamicin-Sensitive Methicillin-Resistant Staphylococcus aureus in Southeast Queensland, Australia. J. Clin. Microbiol.
38: 3926-3931
[Abstract]
[Full Text]