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Journal of Clinical Microbiology, November 1999, p. 3487-3490, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Specific Immunoglobulin E during Maternal, Fetal, and Congenital Toxoplasmosis

I. Villena,1,dagger ,* D. Aubert,1,dagger V. Brodard,1,dagger C. Quereux,2,dagger B. Leroux,3,dagger D. Dupouy,3,dagger G. Remy,4,dagger F. Foudrinier,1,dagger C. Chemla,1,dagger J. E. Gomez-Marin,1,5,dagger and J. M. Pinon1,dagger

Laboratory of Parasitology-Mycology, Team 4 (INSERM U.314), EA 2070, IFR 53,1 Gynecology-Obstetrics Department, Centre Hospitalier Universitaire,2 Pediatrics Department, Centre Hospitalier Universitaire,3 and Infectious Diseases Department, Centre Hospitalier Universitaire,4 Hôpital Maison Blanche, 51092 Reims, France, and Grupo de Patologia Infectiosa, Departamento de Medicina Interna, Hospital San Juan de Dios, Universidad Nacional de Colombia, Santafé de Bogota DC, Colombia5

Received 15 April 1999/Returned for modification 25 June 1999/Accepted 22 July 1999

Toxoplasma immunoglobulin E (IgE) antibodies in 664 serum samples were evaluated by using an immunocapture method with a suspension of tachyzoites prepared in the laboratory in order to evaluate its usefulness in the diagnosis of acute Toxoplasma gondii infection during pregnancy, congenital infection, and progressive toxoplasmosis. IgE antibodies were never detected in sera from seronegative women, from patients with chronic toxoplasma infection, or from infants without congenital toxoplasmosis. In contrast, they were detected in 86.6% of patients with toxoplasmic seroconversion, and compared with IgA and IgM, the short kinetics of IgE was useful to date the infection precisely. For the diagnosis of congenital toxoplasmosis, specific IgE detected was less frequently than IgM or IgA (25 versus 67.3%), but its detection during follow-up of children may be interesting, reflecting an immunological rebound. Finally, IgE was detected early and persisted longer in progressive toxoplasmosis with cervical adenopathies, so it was also a good marker of the evolution of toxoplasma infection.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 51092 Reims, France. Phone: 03-26-78-42-22. Fax: 03-26-78-73-28. E-mail: ivillena{at}chu-reims.fr.

dagger Member of the Reims Toxoplasmosis Group.

Journal of Clinical Microbiology, November 1999, p. 3487-3490, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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