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Journal of Clinical Microbiology, November 1999, p. 3569-3571, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of the Prototype Roche DNA Amplification Kit Incorporating the New SSK145 and SKCC1B Primers in Detection of Human Immunodeficiency Virus Type 1 DNA in Zimbabwe

Lynn S. Zijenah,1,* Jean Humphrey,2 Kussum Nathoo,2 Lucie Malaba,3 Parteson Zvandasara,4 Agnes Mahomva,5 Peter Iliff,2 and Michael T. Mbizvo4,dagger

Departments of Immunology,1 Paediatrics,2 Nutrition,3 and Obstetrics and Gynaecology,4 University of Zimbabwe, and Harare City Health Department,5 Harare, Zimbabwe

Received 2 March 1999/Returned for modification 24 June 1999/Accepted 9 August 1999

We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV-1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.

* Corresponding author. Mailing address: Department of Immunology, University of Zimbabwe Medical School, P.O. Box A178 Avondale, Harare, Zimbabwe. Phone: (263) (4) 791631. Fax: (263) (4) 708015. E-mail: lzijenah{at}healthnet.zw.

dagger Present address: Special Programme of Research, Development and Research Training in Human Reproduction HRP, Geneva, Switzerland.

Journal of Clinical Microbiology, November 1999, p. 3569-3571, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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