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Journal of Clinical Microbiology, November 1999, p. 3586-3589, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Fusarium Species Involved in Human Infections by 28S rRNA Gene Sequencing

C. Hennequin,1,* E. Abachin,1 F. Symoens,2 V. Lavarde,3 G. Reboux,4 N. Nolard,2 and P. Berche1

Service de Microbiologie, Hôpital Necker-Enfants Malades, 75015 Paris,1 Service de Microbiologie, Hôpital Broussais, 75014 Paris,3 and Service de Parasitologie-Mycologie CHU Besançon, 25000 Besançon,4 France, and Institute of Hygiene and Epidemiology Mycology, Brussels, Belgium2

Received 22 March 1999/Returned for modification 10 June 1999/Accepted 28 July 1999

Fusarium spp. have emerged as major opportunistic fungal agents. Since new antifungal agents exhibit variable activity against Fusarium isolates depending on the species, rapid identification at the species level is required. Conventional culture methods are difficult, fastidious, and sometimes inconclusive. In this work, we sequenced a 440-bp fragment encoding the 28S rRNA from 33 Fusarium isolates belonging to six Fusarium species associated with human infections. The data were then analyzed by the neighbor-joining method. By using distance matrix analysis and constructing the phylogram, we could easily distinguish the different species for all but one isolate. The method also allowed differentiation between the closely related genera Acremonium and Cylindrocarpon. In contrast to the case with conventional methods, the results could be obtained within 48 h from a 3-day culture and are independent of mycologist experience, making this method rapid and reliable for identification of Fusarium species isolated from patients.

* Corresponding author. Mailing address: Service de Parasitologie-Mycologie, CHU Amiens, 80054 Amiens Cédex 1, France. Phone: 33-3-22-45-59-75. Fax: 33-3-22-45-56-53. E-mail: chennequin{at}yahoo.com.

Journal of Clinical Microbiology, November 1999, p. 3586-3589, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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