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Journal of Clinical Microbiology, November 1999, p. 3594-3600, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genotypic and Phenotypic Relationships between Clinical and Environmental Isolates of Stenotrophomonas maltophilia

Gabriele Berg,1,* Nicolle Roskot,1 and Kornelia Smalla2

Department of Microbiology, University of Rostock, D-18055 Rostock,1 and Federal Biological Research Centre for Agriculture and Forestry, D-38104 Braunschweig,2 Germany

Received 17 March 1999/Returned for modification 4 May 1999/Accepted 31 July 1999

While the gram-negative bacterium Stenotrophomonas maltophilia is used in biotechnology (e.g., for biological control of plant pathogens and for bioremediation), the number of S. maltophilia diseases in humans has dramatically increased in recent years. A total of 40 S. maltophilia isolates from clinical and environmental sources (plant associated and water) was investigated to determine the intraspecies diversity of the group and to determine whether or not the strains could be grouped based on the source of isolation. The isolates were investigated by phenotypic profiling (enzymatic and metabolic activity and antibiotic resistance patterns) and by molecular methods such as temperature-gradient gel electrophoresis of the 16S rRNA gene fragment, PCR fingerprinting with BOX primers, and pulsed-field gel electrophoresis (PFGE) after digestion with DraI. Results of the various methods revealed high intraspecies diversity. PFGE was the most discriminatory method for typing S. maltophilia when compared to the other molecular methods. The environmental strains of S. maltophilia were highly resistant to antibiotics, and the resistance profile pattern of the strains was not dependent on their source of isolation. Computer-assisted cluster analysis of the phenotypic and genotypic features did not reveal any clustering patterns for either clinical or environmental isolates.

* Corresponding author. Mailing address: University of Rostock, Department of Biology, Microbiology, Gertrudenstrasse 11a, D-18051 ROSTOCK, Germany. Phone: 49 381 494 2049. Fax: 49 381 494 2244. E-mail: gabriele.berg{at}biologie.uni-rostock.de.

Journal of Clinical Microbiology, November 1999, p. 3594-3600, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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