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Journal of Clinical Microbiology, November 1999, p. 3711-3712, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria in a Routine Diagnostic Laboratory

Andreas Roggenkamp,* Mathias W. Hornef, Adelheid Masch, Bettina Aigner, Ingo B. Autenrieth, and Jürgen Heesemann

Max von Pettenkofer-Institute for Hygiene and Microbiology, Ludwig Maximilians University Munich, 80336 Munich, Germany

Received 17 May 1999/Returned for modification 28 June 1999/Accepted 3 August 1999

MB/BacT, BACTEC 460 TB, and Löwenstein-Jensen (LJ) medium were evaluated in parallel for recovery of mycobacteria from 3,700 continuous clinical specimens in a routine laboratory. Mycobacteria were identified from 123 (3.3%) specimens. The recovery rates for all mycobacteria by the different systems were 91.0, 73.0, and 53.6% for BACTEC 460 TB, MB/BacT, and LJ medium, respectively. The recovery rates for Mycobacterium tuberculosis complex were 97.1, 80.2, and 67.6%, respectively. The lack of sensitivity of the MB/BacT system was more pronounced with smear-negative specimens and resulted in a failure to detect three patients with infectious tuberculosis.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut for Hygiene and Microbiology, Ludwig Maximilians University, Munich, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 49-89-51605200. Fax: 49-89-5380584. E-mail: Rogge{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, November 1999, p. 3711-3712, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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