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Journal of Clinical Microbiology, November 1999, p. 3713-3717, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Markers Reveal Exclusively Clonal Reproduction in Trichophyton rubrum

Y. Gräser,* J. Kühnisch, and W. Presber

Department of Microbiology and Hygiene (Charité-Virchow), Humboldt University, Berlin, Germany

Received 14 April 1999/Returned for modification 24 May 1999/Accepted 28 June 1999

Genotypic variability among 96 Trichophyton rubrum strains which displayed different colony morphologies and were collected from four continents was investigated. Twelve markers representing 57 loci were analyzed by PCR fingerprinting, amplified fragment length polymorphism, and random amplified monomorphic DNA markers. Interestingly, none of the methods used revealed any DNA polymorphism, indicating a strictly clonal mode of reproduction and a strong adaptation to human skin.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité-Virchow, Campus Mitte, Humboldt Universität zu Berlin, Dorotheenstr. 96, D-10117 Berlin, Germany. Phone: 49-30-20934799. Fax: 49-30-20934703. E-mail: yvonne.graeser{at}charite.de.

Journal of Clinical Microbiology, November 1999, p. 3713-3717, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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