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Journal of Clinical Microbiology, November 1999, p. 3738-3741, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of a New Commercially Available Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Japanese Encephalitis Infections

Andrea J. Cuzzubbo,1 Timothy P. Endy,2 David W. Vaughn,2,dagger Tom Solomon,3,Dagger Ananda Nisalak,2 Siripen Kalayanarooj,4 Nguyen Minh Dung,5 David Warrilow,6,§ John Aaskov,6 and Peter L. Devine1,*

PanBio Pty. Ltd., Windsor,1 and School of Life Sciences, Queensland University of Technology, Brisbane,6 Queensland, Australia; Department of Virology, Armed Forces Research Institute of Medical Sciences (AFRIMS),2 and Queen Sirikit National Institute of Child Health,4 Bangkok, Thailand; and Wellcome Trust Clinical Research Unit,3 and Paediatric Intensive Care Unit,5 Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, Vietnam

Received 29 April 1999/Returned for modification 6 July 1999/Accepted 9 August 1999

A new commercial enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Japanese encephalitis virus infections showed a sensitivity of 88% with sera and 81% with cerebrospinal fluid and a specificity of 97% with sera from patients with primary and secondary dengue virus infections. Specificity was 100% when samples from nonflavivirus infections were tested.

* Corresponding author. Mailing address: PanBio Pty. Ltd., 116 Lutwyche Rd., Windsor, Queensland 4030, Australia. Phone: 61-7-33571177. Fax: 61-7-33571222. E-mail: peter_devine{at}panbio.com.au.

dagger Present address: Department of Viral Diseases, Walter Reed Army Institute of Research, Washington, DC 20307.

Dagger Present address: Department of Neurological Science, University of Liverpool, Walton Centre for Neurology and Neurosurgery, Fazakerley, Liverpool L9 7LJ, United Kingdom.

§ Present address: Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston, Brisbane 4029, Australia.

Journal of Clinical Microbiology, November 1999, p. 3738-3741, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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