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Journal of Clinical Microbiology, November 1999, p. 3742-3745, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Characterization of a Nonhemolytic Phospholipase C Gene from Burkholderia pseudomallei

Sunee Korbsrisate,1,* Nuttiga Suwanasai,1 Amornrut Leelaporn,2 Takayuki Ezaki,3 Yoshiaki Kawamura,3 and Suttipant Sarasombath1

Departments of Immunology1 and Microbiology,2 Faculty of Medicine Siriraj Hospital, Bangkok 10700, Thailand, and Department of Microbiology, Gifu University School of Medicine, Gifu 500, Japan3

Received 17 March 1999/Returned for modification 14 May 1999/Accepted 31 July 1999

We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein.

* Corresponding author. Mailing address: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Rd., Bangkok 10700, Thailand. Phone: (66-2) 419-7066. Fax: (66-2) 418-1636. E-mail: grsks{at}mahidol.ac.th.

Journal of Clinical Microbiology, November 1999, p. 3742-3745, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Korbsrisate, S., Tomaras, A. P., Damnin, S., Ckumdee, J., Srinon, V., Lengwehasatit, I., Vasil, M. L., Suparak, S. (2007). Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomallei. Microbiology 153: 1907-1915 [Abstract] [Full Text]  
  • Tuanyok, A., Tom, M., Dunbar, J., Woods, D. E. (2006). Genome-Wide Expression Analysis of Burkholderia pseudomallei Infection in a Hamster Model of Acute Melioidosis.. Infect. Immun. 74: 5465-5476 [Abstract] [Full Text]  
  • Cheng, A. C., Currie, B. J. (2005). Melioidosis: Epidemiology, Pathophysiology, and Management. Clin. Microbiol. Rev. 18: 383-416 [Abstract] [Full Text]  


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