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Journal of Clinical Microbiology, November 1999, p. 3761-3763, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Recovery of Mycobacteria from Patients with Cystic Fibrosis

Franz-Christoph Bange,* Philip Kirschner, and Eric C. Böttger

Institute of Medical Microbiology, Medical School Hannover, 30625 Hannover, Germany

Received 19 February 1999/Returned for modification 10 July 1999/Accepted 31 July 1999

Despite decontamination, overgrowth by pseudomonads renders cultural isolation of mycobacteria from respiratory specimens of patients with cystic fibrosis (CF) difficult or impossible. We performed a prospective study by comparing levels of reduction of overgrowth and recovery of mycobacteria using either pretreatment with N-acetyl-L-cysteine (NALC)-NaOH alone or pretreatment with NALC-NaOH and then with oxalic acid. From 406 specimens of 148 CF patients, 11 specimens were positive for mycobacteria, 5 of which grew mycobacteria after decontamination by either procedure. Three specimens grew mycobacteria only after decontamination with NALC-NaOH, whereas three specimens grew mycobacteria only after treatment with NALC-NaOH followed by oxalic acid but were overgrown after decontamination with NALC-NaOH. Thus, inactivation of mycobacteria by the more aggressive oxalic acid treatment offsets its beneficial effect of reducing the proportion of cultures overgrown with microorganisms other than mycobacteria.

* Corresponding author. Mailing address: Institute für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Phone: 49-511-532-4352. Fax: 49-511-532-4366. E-mail: bange{at}mikrobio.mh-hannover.de.

Journal of Clinical Microbiology, November 1999, p. 3761-3763, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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