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Journal of Clinical Microbiology, November 1999, p. 3769-3773, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of Extended-Spectrum beta -Lactamase-Producing Salmonella typhimurium by Phenotypic and Genotypic Typing Methods

Rajaa Ait Mhand,1 Naima Brahimi,2 Najat Moustaoui,1 Naima El Mdaghri,1 Hamid Amarouch,3 Francine Grimont,4 Edouard Bingen,2 and Mohamed Benbachir1,*

Microbiology Laboratory, Ibn Rochd University Hospital,1 and Department of Biology, University Hassan II,3 Casablanca, Morocco, and Microbiology Laboratory of Hospital Robert-Debré,2 and French National Center for Enteric Molecular Typing, Pasteur Institute,4 Paris, France

Received 16 February 1999/Returned for modification 26 April 1999/Accepted 22 July 1999

During 1994, 10 isolates of extended-spectrum beta -lactamase-producing Salmonella typhimurium were recovered from children transferred to our hospital from two different centers. Two additional isolates were recovered from two nurses from one of these centers. The aim of this study was to determine if there is any relationship between these isolates. The characterization was done by phenotypic and genotypic methods: biotyping, phage typing, antibiotic susceptibility pattern determination, plasmid analysis, ribotyping (by the four endonucleases EcoRI, SmaI, BglII, and PvuII), pulsed-field gel electrophoresis (PFGE) of genome macrorestriction patterns with XbaI, and randomly amplified polymorphic DNA (RAPD) pattern determination (with the three primers 217 d2, B1, and A3). The same biotype, the same serotype, and an identical antibiotype were found. All isolates were resistant to oxyimino-beta -lactams, gentamicin, tobramycin, and sulfamethoxazole-trimethoprim. All isolates showed an indistinguishable pattern by ribotyping and very similar patterns by PFGE and RAPD. The overall results indicated the spread of a closely related strain of S. typhimurium in children and nurses.

* Corresponding author. Mailing address: Faculté de Médecine, Laboratoire de Microbiologie, 19 rue Tarik Bnouzyad, Casablanca BP 9154, Morocco. Phone and fax: (212-2) 26-90-57. E-mail: mohamed.benbachir{at}fmp-uh2c.ac.ma.

Journal of Clinical Microbiology, November 1999, p. 3769-3773, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Nair, S., Lin, T. K., Pang, T., Altwegg, M. (2002). Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis. J. Clin. Microbiol. 40: 2346-2351 [Abstract] [Full Text]  
  • AitMhand, R., Soukri, A., Moustaoui, N., Amarouch, H., ElMdaghri, N., Sirot, D., Benbachir, M. (2002). Plasmid-mediated TEM-3 extended-spectrum {beta}-lactamase production in Salmonella typhimurium in Casablanca. J Antimicrob Chemother 49: 169-172 [Abstract] [Full Text]  


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