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Journal of Clinical Microbiology, December 1999, p. 3800-3803, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification and PCR-Restriction Fragment Length Polymorphism Analysis of a Variant of the Ibaraki Virus from Naturally Infected Cattle and Aborted Fetuses in Japan

Seiichi Ohashi,1,* Kazuo Yoshida,1 Youichirou Watanabe,2 and Tomoyuki Tsuda1

Laboratory of Clinical Virology, Kyushu Research Station, National Institute of Animal Health, 2702, Chuzan, Kagoshima 891-0105,1 and Central Livestock Hygiene Service Center, Kamifukumoto-cho, 5500, Kagoshima 891-0116,2 Japan

Received 15 March 1999/Returned for modification 7 June 1999/Accepted 8 September 1999

One hundred fourteen field isolates of the Ibaraki virus (IBAV), a member of the epizootic hemorrhagic disease virus serotype 2 (EHDV-2), were isolated from blood samples of affected and apparently healthy cattle and Culicoides biting midges and from blood samples of dams and internal organs of aborted fetuses during an outbreak of Ibaraki disease in the southern part of Japan in 1997. In this outbreak, 242 cattle showed typical symptoms of the disease, and several hundred dams had miscarriages or stillbirths. The viruses that induced typical Ibaraki disease and reproductive problems among cattle were identical and were antigenically closely related to but distinct from previous isolates of IBAV and EHDV-2. The virus was considered to be a putative agent of this outbreak. Reverse transcription-PCR based on segment 3 of the RNA genome of EHDV-2 and restriction fragment length polymorphism analysis of the PCR products were conducted to compare the genomes of the viruses. The results suggested that the virus isolated in 1997 was a variant of IBAV and might be exotic.

* Corresponding author. Mailing address: Laboratory of Clinical Virology, Kyushu Research Station, National Institute of Animal Health, 2702, Chuzan, Kagoshima 891-0105, Japan. Phone: 81-99-268-2078. Fax: 81-99-268-3088. E-mail: ohashis{at}sat.affrc.go.jp.

Journal of Clinical Microbiology, December 1999, p. 3800-3803, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.