Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and alpha -Methyl-D-Glucoside as Determined with the API 20C AUX and Vitek YBC Systems

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Journal of Clinical Microbiology, December 1999, p. 3804-3808, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and $alpha$ -Methyl-D-Glucoside as Determined with the API 20C AUX and Vitek YBC Systems

A. C. Gales,¹^,²^,^* M. A. Pfaller,¹ A. K. Houston,¹ S. Joly,³ D. J. Sullivan,⁴ D. C. Coleman,⁴ and D. R. Soll³

Departments of Pathology¹ and Biology,³ University of Iowa, Iowa City, Iowa; Department of Oral Medicine and Pathology, School of Dental Science, and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland⁴; and Department of Medicine, Division of Infectious Diseases, Universidade Federal de Sao Paulo, Sao Paulo, Brazil²

Received 3 May 1999/Returned for modification 28 June 1999/Accepted 20 August 1999

To have a better understanding of the role of Candida dubliniensis in clinical infections, it is essential that microbiologylaboratories can identify this species rapidly and accuratelyin clinical specimens. C. dubliniensis has been reported to lackthe ability to utilize xylose (XYL) and $alpha$ -methyl-D-glucoside (MDG)and to grow poorly or not at all at 45°C, whereas Candida albicansisolates utilize XYL and MDG and usually grow well at 45°C. Wetested 66 isolates of C. dubliniensis and 100 isolates of C. albicanswith both the API 20C AUX and Vitek YBC systems to evaluate theability of the XYL and MDG tests contained within each of thesesystems to distinguish between the two species. The ability togrow at 45°C was also examined. None of the C. dubliniensis isolatesgrew at 45°C, and 23 of 100 C. albicans isolates (23%) exhibitedpoor or no growth at 45°C. The XYL and MDG tests contained withinthe API 20C AUX system were both negative for all 66 C. dubliniensisisolates and were positive for 98 (XYL) and 56 (MDG) of the 100C. albicans isolates. With the Vitek system, 64 of 66 C. dubliniensisisolates (97.0%) were XYL negative and 63 (95.0%) were MDG negative.Conversely, 96 of 100 C. albicans isolates (96.0%) were XYL positiveand 100 (100.0%) were MDG positive with the Vitek system. Clinicalmicrobiology laboratories could use lack of growth at 45°C anda negative XYL test with either the API 20C AUX or Vitek yeastidentification system to provide a presumptive identificationof C. dubliniensis. A negative MDG test result with either systemwould also be helpful but may misclassify C. albicans as C. dubliniensis,especially when the API 20C AUX system isused.

^* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of IowaCollege of Medicine, Iowa City, IA 52242. Phone: (319) 355-8172.Fax: (319) 356-4916. E-mail: galesa{at}mail.medadmin.uiowa.edu.

Journal of Clinical Microbiology, December 1999, p. 3804-3808, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and -Methyl-D-Glucoside as Determined with the API 20C AUX and Vitek YBC Systems

Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and $alpha$ -Methyl-D-Glucoside as Determined with the API 20C AUX and Vitek YBC Systems