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Journal of Clinical Microbiology, December 1999, p. 3835-3843, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Species and Genotypic Diversities and Similarities of Pathogenic Yeasts Colonizing Womendagger

Jianping Xu,1,* Cynthia M. Boyd,1 Elizabeth Livingston,2 Wieland Meyer,3 John F. Madden,4 and Thomas G. Mitchell1

Department of Microbiology,1 Division of Maternal and Fetal Medicine, Department of Obstetrics and Gynecology,2 and Clinical Microbiology Laboratory, Department of Pathology,4 Duke University Medical Center, Durham, North Carolina 27710, and Center for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Sydney, New South Wales 2145, Australia3

Received 11 June 1999/Returned for modification 6 August 1999/Accepted 23 August 1999

We examined the patterns of strain relatedness among pathogenic yeasts from within and among groups of women to determine whether there were significant associations between genotype and host condition or body site. A total of 80 yeast strains were isolated, identified, and genotyped from 49 female volunteers, who were placed in three groups: (i) 19 women with AIDS, (ii) 11 pregnant women without human immunodeficiency virus (HIV) infection, and (iii) 19 women who were neither pregnant nor infected with HIV. Seven yeast species were recovered, including 59 isolates of Candida albicans, 9 isolates of Candida parapsilosis, 5 isolates of Candida krusei, 3 isolates of Candida glabrata, 2 isolates of Saccharomyces cerevisiae, and 1 isolate each of Candida tropicalis and Candida lusitaniae. Seventy unique genotypes were identified by PCR fingerprinting with the M13 core sequence and by random amplified polymorphic DNA analysis. Of the nine shared genotypes, isolates from three different hosts were of one genotype and pairs of strains from different body sites of the same host shared each of the other eight genotypes. Genetic similarities among groups of strains were calculated and compared. We found no significant difference in the patterns of relatedness of strains from the three body sites (oral cavity, vagina, and rectum), regardless of host conditions. The yeast microflora of all three host groups had similar species and genotypic diversities. Furthermore, a single host can be colonized with multiple species or multiple genotypes of the same species at the same or different body sites, indicating dynamic processes of yeast colonization on women.

* Corresponding author. Mailing address: Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-9096. Fax: (919) 681-8911. E-mail: jpxu{at}acpub.duke.edu.

dagger This is a contribution of the Duke University Mycology Research Unit.

Journal of Clinical Microbiology, December 1999, p. 3835-3843, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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