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Journal of Clinical Microbiology, December 1999, p. 3835-3843, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Species and Genotypic Diversities and Similarities
of Pathogenic Yeasts Colonizing Women
Jianping
Xu,1,*
Cynthia M.
Boyd,1
Elizabeth
Livingston,2
Wieland
Meyer,3
John F.
Madden,4 and
Thomas G.
Mitchell1
Department of
Microbiology,1 Division of Maternal and
Fetal Medicine, Department of Obstetrics and
Gynecology,2 and Clinical Microbiology
Laboratory, Department of Pathology,4 Duke
University Medical Center, Durham, North Carolina 27710, and
Center for Infectious Diseases and Microbiology, The
University of Sydney, Westmead Hospital, Sydney, New South Wales
2145, Australia3
Received 11 June 1999/Returned for modification 6 August
1999/Accepted 23 August 1999
We examined the patterns of strain relatedness among pathogenic
yeasts from within and among groups of women to determine whether there
were significant associations between genotype and host condition or
body site. A total of 80 yeast strains were isolated, identified, and
genotyped from 49 female volunteers, who were placed in three groups:
(i) 19 women with AIDS, (ii) 11 pregnant women without human
immunodeficiency virus (HIV) infection, and (iii) 19 women who were
neither pregnant nor infected with HIV. Seven yeast species were
recovered, including 59 isolates of Candida albicans, 9 isolates of Candida parapsilosis, 5 isolates of
Candida krusei, 3 isolates of Candida glabrata,
2 isolates of Saccharomyces cerevisiae, and 1 isolate each
of Candida tropicalis and Candida lusitaniae.
Seventy unique genotypes were identified by PCR fingerprinting with the
M13 core sequence and by random amplified polymorphic DNA analysis. Of
the nine shared genotypes, isolates from three different hosts were of
one genotype and pairs of strains from different body sites of the same
host shared each of the other eight genotypes. Genetic similarities
among groups of strains were calculated and compared. We found no
significant difference in the patterns of relatedness of strains from
the three body sites (oral cavity, vagina, and rectum), regardless of
host conditions. The yeast microflora of all three host groups had
similar species and genotypic diversities. Furthermore, a single host
can be colonized with multiple species or multiple genotypes of the
same species at the same or different body sites, indicating dynamic
processes of yeast colonization on women.
*
Corresponding author. Mailing address: Department of
Microbiology, Box 3020, Duke University Medical Center, Durham, NC
27710. Phone: (919) 684-9096. Fax: (919) 681-8911. E-mail:
jpxu{at}acpub.duke.edu.
This is a contribution of the Duke University Mycology Research Unit.
Journal of Clinical Microbiology, December 1999, p. 3835-3843, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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