This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xu, J.
Right arrow Articles by Mitchell, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, J.
Right arrow Articles by Mitchell, T. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 1999, p. 3835-3843, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Species and Genotypic Diversities and Similarities of Pathogenic Yeasts Colonizing Womendagger

Jianping Xu,1,* Cynthia M. Boyd,1 Elizabeth Livingston,2 Wieland Meyer,3 John F. Madden,4 and Thomas G. Mitchell1

Department of Microbiology,1 Division of Maternal and Fetal Medicine, Department of Obstetrics and Gynecology,2 and Clinical Microbiology Laboratory, Department of Pathology,4 Duke University Medical Center, Durham, North Carolina 27710, and Center for Infectious Diseases and Microbiology, The University of Sydney, Westmead Hospital, Sydney, New South Wales 2145, Australia3

Received 11 June 1999/Returned for modification 6 August 1999/Accepted 23 August 1999

We examined the patterns of strain relatedness among pathogenic yeasts from within and among groups of women to determine whether there were significant associations between genotype and host condition or body site. A total of 80 yeast strains were isolated, identified, and genotyped from 49 female volunteers, who were placed in three groups: (i) 19 women with AIDS, (ii) 11 pregnant women without human immunodeficiency virus (HIV) infection, and (iii) 19 women who were neither pregnant nor infected with HIV. Seven yeast species were recovered, including 59 isolates of Candida albicans, 9 isolates of Candida parapsilosis, 5 isolates of Candida krusei, 3 isolates of Candida glabrata, 2 isolates of Saccharomyces cerevisiae, and 1 isolate each of Candida tropicalis and Candida lusitaniae. Seventy unique genotypes were identified by PCR fingerprinting with the M13 core sequence and by random amplified polymorphic DNA analysis. Of the nine shared genotypes, isolates from three different hosts were of one genotype and pairs of strains from different body sites of the same host shared each of the other eight genotypes. Genetic similarities among groups of strains were calculated and compared. We found no significant difference in the patterns of relatedness of strains from the three body sites (oral cavity, vagina, and rectum), regardless of host conditions. The yeast microflora of all three host groups had similar species and genotypic diversities. Furthermore, a single host can be colonized with multiple species or multiple genotypes of the same species at the same or different body sites, indicating dynamic processes of yeast colonization on women.

* Corresponding author. Mailing address: Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-9096. Fax: (919) 681-8911. E-mail: jpxu{at}acpub.duke.edu.

dagger This is a contribution of the Duke University Mycology Research Unit.

Journal of Clinical Microbiology, December 1999, p. 3835-3843, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Antonopoulou, S., Aoun, M., Alexopoulos, E. C., Baka, S., Logothetis, E., Kalambokas, T., Zannos, A., Papadias, K., Grigoriou, O., Kouskouni, E., Velegraki, A. (2009). Fenticonazole Activity Measured by the Methods of the European Committee on Antimicrobial Susceptibility Testing and CLSI against 260 Candida Vulvovaginitis Isolates from Two European Regions and Annotations on the Prevalent Genotypes. Antimicrob. Agents Chemother. 53: 2181-2184 [Abstract] [Full Text]  
  • Bougnoux, M.-E., Diogo, D., Francois, N., Sendid, B., Veirmeire, S., Colombel, J. F., Bouchier, C., Van Kruiningen, H., d'Enfert, C., Poulain, D. (2006). Multilocus Sequence Typing Reveals Intrafamilial Transmission and Microevolutions of Candida albicans Isolates from the Human Digestive Tract.. J. Clin. Microbiol. 44: 1810-1820 [Abstract] [Full Text]  
  • Lan, L., Xu, J. (2006). Multiple gene genealogical analyses suggest divergence and recent clonal dispersal in the opportunistic human pathogen Candida guilliermondii.. Microbiology 152: 1539-1549 [Abstract] [Full Text]  
  • Chong, P. P., Lee, Y. L., Tan, B. C., Ng, K. P. (2003). Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia. J Med Microbiol 52: 657-666 [Abstract] [Full Text]  
  • Montour, L., Tey, R., Xu, J. (2003). Isolation of Candida dubliniensis in an Aboriginal Community in Ontario, Canada. J. Clin. Microbiol. 41: 3423-3426 [Abstract] [Full Text]  
  • Lott, T. J., Fundyga, R. E., Brandt, M. E., Harrison, L. H., Sofair, A. N., Hajjeh, R. A., Warnock, D. W. (2003). Stability of Allelic Frequencies and Distributions of Candida albicans Microsatellite Loci from U.S. Population-Based Surveillance Isolates. J. Clin. Microbiol. 41: 1316-1321 [Abstract] [Full Text]  
  • Rath, P.-M., Petermeier, K., Verweij, P. E., Ansorg, R. (2002). Differentiation of Aspergillus ustus Strains by Random Amplification of Polymorphic DNA. J. Clin. Microbiol. 40: 2231-2233 [Abstract] [Full Text]  
  • Bacci, A., Montagnoli, C., Perruccio, K., Bozza, S., Gaziano, R., Pitzurra, L., Velardi, A., d'Ostiani, C. F., Cutler, J. E., Romani, L. (2002). Dendritic Cells Pulsed with Fungal RNA Induce Protective Immunity to Candida albicans in Hematopoietic Transplantation. J. Immunol. 168: 2904-2913 [Abstract] [Full Text]  
  • Francois, F., Noel, T., Pepin, R., Brulfert, A., Chastin, C., Favel, A., Villard, J. (2001). Alternative Identification Test Relying upon Sexual Reproductive Abilities of Candidalusitaniae Strains Isolated from Hospitalized Patients. J. Clin. Microbiol. 39: 3906-3914 [Abstract] [Full Text]  
  • ZOUHAIR, R., DEFONTAINE, A., OLLIVIER, C., CIMON, B., SYMOENS, F., HALLET, J.-N., DEUNFF, J., BOUCHARA, J.-P. (2001). Typing of Scedosporium apiospermum by multilocus enzyme electrophoresis and random amplification of polymorphic DNA. J Med Microbiol 50: 925-932 [Abstract] [Full Text]  
  • Lott, T. J., Effat, M. M. (2001). Evidence for a more recently evolved clade within a Candida albicans North American population. Microbiology 147: 1687-1692 [Abstract] [Full Text]  
  • Luu, L. N., Cowen, L. E., Sirjusingh, C., Kohn, L. M., Anderson, J. B. (2001). Multilocus Genotyping Indicates that the Ability To Invade the Bloodstream Is Widespread among Candida albicans Isolates. J. Clin. Microbiol. 39: 1657-1660 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»