Journal of Clinical Microbiology, December 1999, p. 3851-3855, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Department of Environmental & Resource Sciences, University of Nevada, Reno, Nevada 895571; LAC + University of Southern California Medical Center, Los Angeles, California 900332; and BIOLOG, Inc., Hayward, California 945453
Received 10 June 1999/Returned for modification 10 July 1999/Accepted 31 August 1999
The major clinical problem for patients with cystic fibrosis (CF)
is progressive loss of pulmonary function, usually due to chronic
bacterial infections. A patient with CF and a lung transplant was
severely infected with a previously unidentified gram-negative bacterium. We isolated this organism (strain DS15158) from the patient
and characterized it by phylogenetic analysis of the small-subunit rRNA
and biochemically by the BIOLOG GN MicroPlate assay, fatty acid
analysis, and various standard laboratory tests. No close match to any
other organism could be found. Isolate DS15158 represents a new
genus-level divergence within the bacterial subdivision
-Proteobacteria on the basis of the 16S rRNA gene analysis.
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