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Journal of Clinical Microbiology, December 1999, p. 3851-3855, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Novel Bacterium Isolated from a Lung Transplant Patient with Cystic Fibrosis

Christian Pitulle,1 Diane M. Citron,2 Barry Bochner,3 Richard Barbers,2 and Maria D. Appleman2,*

Department of Environmental & Resource Sciences, University of Nevada, Reno, Nevada 895571; LAC + University of Southern California Medical Center, Los Angeles, California 900332; and BIOLOG, Inc., Hayward, California 945453

Received 10 June 1999/Returned for modification 10 July 1999/Accepted 31 August 1999

The major clinical problem for patients with cystic fibrosis (CF) is progressive loss of pulmonary function, usually due to chronic bacterial infections. A patient with CF and a lung transplant was severely infected with a previously unidentified gram-negative bacterium. We isolated this organism (strain DS15158) from the patient and characterized it by phylogenetic analysis of the small-subunit rRNA and biochemically by the BIOLOG GN MicroPlate assay, fatty acid analysis, and various standard laboratory tests. No close match to any other organism could be found. Isolate DS15158 represents a new genus-level divergence within the bacterial subdivision alpha -Proteobacteria on the basis of the 16S rRNA gene analysis.

* Corresponding author. Mailing address: LAC + USC Medical Center, 1200 N. State St., Room 2014, Los Angeles, CA 90033. Phone: (323) 226-7016. Fax: (323) 226-4075. E-mail: mapplema{at}hsc.usc.edu.

Journal of Clinical Microbiology, December 1999, p. 3851-3855, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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