This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lawson, A. J.
Right arrow Articles by Stanley, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lawson, A. J.
Right arrow Articles by Stanley, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 1999, p. 3860-3864, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Large-Scale Survey of Campylobacter Species in Human Gastroenteritis by PCR and PCR-Enzyme-Linked Immunosorbent Assay

A. J. Lawson, J. M. J. Logan, G. L. O'neill, M. Desai, and J. Stanley*

Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 4 June 1999/Returned for modification 17 August 1999/Accepted 13 September 1999

A PCR-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening PCRs and species identification by PCR-enzyme-linked immunosorbent assay. This was applied to DNA extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven regional Public Health Laboratories in England and Wales over a 2-year period. The sending laboratories had cultured "Campylobacter spp." from 464 samples. The PCR methodologies detected 492 Campylobacter-positive samples, and the combination of culture and PCR yielded 543 Campylobacter-positive samples. There was identity (overlap) for 413 samples, but 79 PCR-positive samples were culture negative, and 51 culture-positive samples were PCR negative. While there was no statistically significant difference between PCR and culture in detection of C. jejuni-C. coli (PCR, 478 samples; culture, 461 samples), PCR provided unique data about mixed infections and non-C. jejuni and non- C. coli campylobacters. Mixed infections with C. jejuni and C. coli were found in 19 samples, and mixed infection with C. jejuni and C. upsaliensis was found in one sample; this was not apparent from culture. Eleven cases of gastroenteritis were attributed to C. upsaliensis by PCR, three cases were attributed to C. hyointestinalis, and one case was attributed to C. lari. This represents the highest incidence of C. hyointestinalis yet reported from human gastroenteritis, while the low incidence of C. lari suggests that it is less important in this context.

* Corresponding author. Mailing address: Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 0208 2004400, ext. 3090. Fax: 0208 2001569. E-mail: sevenwoods{at}hotmail.com.

Journal of Clinical Microbiology, December 1999, p. 3860-3864, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • O'Leary, J., Corcoran, D., Lucey, B. (2009). Comparison of the EntericBio Multiplex PCR System with Routine Culture for Detection of Bacterial Enteric Pathogens. J. Clin. Microbiol. 47: 3449-3453 [Abstract] [Full Text]  
  • Westgarth, C., Porter, C. J., Nicolson, L., Birtles, R. J., Williams, N. J., Hart, C. A., Pinchbeck, G. L., Gaskell, R. M., Christley, R. M., Dawson, S. (2009). Risk factors for the carriage of Campylobacter upsaliensis by dogs in a community in Cheshire. Vet Rec. 165: 526-530 [Abstract] [Full Text]  
  • Yamazaki, W., Taguchi, M., Ishibashi, M., Kitazato, M., Nukina, M., Misawa, N., Inoue, K. (2008). Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Campylobacter jejuni and Campylobacter coli. J Med Microbiol 57: 444-451 [Abstract] [Full Text]  
  • Schuurman, T., de Boer, R. F., van Zanten, E., van Slochteren, K. R., Scheper, H. R., Dijk-Alberts, B. G., Moller, A. V. M., Kooistra-Smid, A. M. D. (2007). Feasibility of a Molecular Screening Method for Detection of Salmonella enterica and Campylobacter jejuni in a Routine Community-Based Clinical Microbiology Laboratory. J. Clin. Microbiol. 45: 3692-3700 [Abstract] [Full Text]  
  • Axelsson-Olsson, D., Ellstrom, P., Waldenstrom, J., Haemig, P. D., Brudin, L., Olsen, B. (2007). Acanthamoeba-Campylobacter Coculture as a Novel Method for Enrichment of Campylobacter Species. Appl. Environ. Microbiol. 73: 6864-6869 [Abstract] [Full Text]  
  • Yamazaki-Matsune, W., Taguchi, M., Seto, K., Kawahara, R., Kawatsu, K., Kumeda, Y., Kitazato, M., Nukina, M., Misawa, N., Tsukamoto, T. (2007). Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. J Med Microbiol 56: 1467-1473 [Abstract] [Full Text]  
  • Lund, M., Nordentoft, S., Pedersen, K., Madsen, M. (2004). Detection of Campylobacter spp. in Chicken Fecal Samples by Real-Time PCR. J. Clin. Microbiol. 42: 5125-5132 [Abstract] [Full Text]  
  • Keramas, G., Bang, D. D., Lund, M., Madsen, M., Bunkenborg, H., Telleman, P., Christensen, C. B. V. (2004). Use of Culture, PCR Analysis, and DNA Microarrays for Detection of Campylobacter jejuni and Campylobacter coli from Chicken Feces. J. Clin. Microbiol. 42: 3985-3991 [Abstract] [Full Text]  
  • Iijima, Y., Asako, N. T., Aihara, M., Hayashi, K. (2004). Improvement in the detection rate of diarrhoeagenic bacteria in human stool specimens by a rapid real-time PCR assay. J Med Microbiol 53: 617-622 [Abstract] [Full Text]  
  • Hald, B., Pedersen, K., Waino, M., Jorgensen, J. C., Madsen, M. (2004). Longitudinal Study of the Excretion Patterns of Thermophilic Campylobacter spp. in Young Pet Dogs in Denmark. J. Clin. Microbiol. 42: 2003-2012 [Abstract] [Full Text]  
  • Maher, M., Finnegan, C., Collins, E., Ward, B., Carroll, C., Cormican, M. (2003). Evaluation of Culture Methods and a DNA Probe-Based PCR Assay for Detection of Campylobacter Species in Clinical Specimens of Feces. J. Clin. Microbiol. 41: 2980-2986 [Abstract] [Full Text]  
  • Inglis, G. D., Kalischuk, L. D. (2003). Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces. Appl. Environ. Microbiol. 69: 3435-3447 [Abstract] [Full Text]  
  • Hong, Y., Berrang, M. E., Liu, T., Hofacre, C. L., Sanchez, S., Wang, L., Maurer, J. J. (2003). Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay. Appl. Environ. Microbiol. 69: 3492-3499 [Abstract] [Full Text]  
  • Sails, A. D., Fox, A. J., Bolton, F. J., Wareing, D. R. A., Greenway, D. L. A. (2003). A Real-Time PCR Assay for the Detection of Campylobacter jejuni in Foods after Enrichment Culture. Appl. Environ. Microbiol. 69: 1383-1390 [Abstract] [Full Text]  
  • Kulkarni, S P, Lever, S, Logan, J M J, Lawson, A J, Stanley, J, Shafi, M S (2002). Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods. J. Clin. Pathol. 55: 749-753 [Abstract] [Full Text]  
  • Sails, A. D., Bolton, F. J., Fox, A. J., Wareing, D. R. A., Greenway, D. L. A. (2002). Detection of Campylobacter jejuni and Campylobacter coli in Environmental Waters by PCR Enzyme-Linked Immunosorbent Assay. Appl. Environ. Microbiol. 68: 1319-1324 [Abstract] [Full Text]  
  • Fox, J G (2002). The non-H pylori helicobacters: their expanding role in gastrointestinal and systemic diseases. Gut 50: 273-283 [Abstract] [Full Text]  
  • MISAWA, N., KAWASHIMA, K., KAWAMOTO, H., KONDO, F. (2002). Development of a combined filtration-enrichment culture followed by a one-step duplex PCR technique for the rapid detection of Campylobacter jejuni and C. coli in human faecal samples. J Med Microbiol 51: 86-89 [Abstract] [Full Text]  
  • Collins, E., Glennon, M., Hanley, S., Murray, A.-M., Cormican, M., Smith, T., Maher, M. (2001). Evaluation of a PCR/DNA Probe Colorimetric Membrane Assay for Identification of Campylobacter spp. in Human Stool Specimens. J. Clin. Microbiol. 39: 4163-4165 [Abstract] [Full Text]  
  • Shen, Z., Feng, Y., Dewhirst, F. E., Fox, J. G. (2001). Coinfection of Enteric Helicobacter spp. and Campylobacter spp. in Cats. J. Clin. Microbiol. 39: 2166-2172 [Abstract] [Full Text]  
  • Logan, J. M. J., Edwards, K. J., Saunders, N. A., Stanley, J. (2001). Rapid Identification of Campylobacter spp. by Melting Peak Analysis of Biprobes in Real-Time PCR. J. Clin. Microbiol. 39: 2227-2232 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»