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Journal of Clinical Microbiology, December 1999, p. 3925-3927, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Diagnosis of Tuberculous Meningitis by a Dot Immunobinding Assay To Detect Mycobacterial Antigen in Cerebrospinal Fluid Specimens

M. G. Sumi,1 A. Mathai,1 C. Sarada,2 and V. V. Radhakrishnan1,*

Departments of Pathology1 and Neurology,2 Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695 011, Kerala, India

Received 14 May 1999/Returned for modification 24 June 1999/Accepted 2 August 1999

In the present prospective study, a dot immunobinding assay (Dot-Iba) was standardized to measure the circulating mycobacterial antigen in cerebrospinal fluid (CSF) specimens for the laboratory diagnosis of tuberculous meningitis (TBM). Immunoglobulin G antibody specific for Mycobacterium tuberculosis in a CSF specimen from a patient with culture-proven TBM was isolated and was coupled with activated cyanogen bromide-Sepharose 4B. By immunosorbent affinity chromatography, a 14-kDa antigen was isolated from the culture filtrate of M. tuberculosis. Antibody to the 14-kDa mycobacterial antigen was raised in rabbits. The Dot-Iba in this study gave no false-positive results with CSF specimens from patients with nontuberculous neurological diseases. The assay gave positive results for all five patients with culture-proven TBM. The Dot-Iba described in the present report is simple, rapid, sensitive, specific, and, more importantly, suitable for routine application in laboratories in developing countries.

* Corresponding author. Mailing address: Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram-695 011, Kerala, India. Phone: (91)-471-524508. Fax: (91)-471-446433.

Journal of Clinical Microbiology, December 1999, p. 3925-3927, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rock, R. B., Olin, M., Baker, C. A., Molitor, T. W., Peterson, P. K. (2008). Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects. Clin. Microbiol. Rev. 21: 243-261 [Abstract] [Full Text]  
  • Sumi, M. G., Mathai, A., Reuben, S., Sarada, C., Radhakrishnan, V. V. (2002). Immunocytochemical Method for Early Laboratory Diagnosis of Tuberculous Meningitis. CVI 9: 344-347 [Abstract] [Full Text]  


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