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Journal of Clinical Microbiology, December 1999, p. 3952-3956, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Acute-Phase-Specific Heptapeptide Epitope for Diagnosis of Parvovirus B19 Infection

Leena Kaikkonen,1 Hilkka Lankinen,1 Irene Harjunpää,1 Kati Hokynar,1 Maria Söderlund-Venermo,1 Christian Oker-Blom,2 Lea Hedman,1 and Klaus Hedman1,*

Department of Virology, Haartman Institute and HUCH Diagnostic, University of Helsinki, Helsinki,1 and VTT Biotechnology and Food Research, VTT, Espoo,2 Finland

Received 20 April 1999/Returned for modification 5 July 1999/Accepted 16 August 1999

The major capsid protein VP2 of human parvovirus B19, when studied in a denatured form exhibiting linear epitopes, is recognized exclusively by immunoglobulin G (IgG) antibodies of patients with acute or recent B19 infection. By contrast, conformational epitopes of VP2 are recognized both by IgG of the acute phase and by IgG of past immunity. In order to localize the VP2 linear epitope(s) specific for acute-phase IgG, the entire B19 capsid protein sequence was mapped by peptide scanning using well-characterized acute-phase and control sera. A unique heptapeptide epitope showing strong and selective reactivity with the acute-phase IgG was detected and characterized. By using this linear epitope (VP2 amino acids 344 to 350) and virus-like particles exhibiting conformational VP2 epitopes, an innovative approach, second-generation epitope-typing enzyme immunoassay, was set up for improved diagnosis of primary infections by human parvovirus B19.

* Corresponding author. Mailing address: Haartman Institute and HUCH Diagnostic, Department of Virology, P.O. Box 21 (Haartmaninkatu 3), FIN-00014 University of Helsinki, Finland. Phone: 358-50-5249 086. Fax: 358-9-1912 6491. E-mail: klaus.hedman{at}helsinki.fi.

Journal of Clinical Microbiology, December 1999, p. 3952-3956, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1999 by the American Society for Microbiology. All rights reserved.