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Journal of Clinical Microbiology, December 1999, p. 4005-4011, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

rRNA Gene Internal Transcribed Spacer 1 and 2 Sequences of Asexual, Anthropophilic Dermatophytes Related to Trichophyton rubrum

R. C. Summerbell,1,2,* R. A. Haugland,3 A. Li,1 and A. K. Gupta4

Laboratories Branch, Ontario Ministry of Health,1 and Department of Laboratory Medicine and Pathobiology2 and Division of Dermatology, Department of Medicine,4 University of Toronto, Toronto, Ontario, Canada, and National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio3

Received 18 May 1999/Returned for modification 30 June 1999/Accepted 18 August 1999

The ribosomal region spanning the two internal transcribed spacer (ITS) regions and the 5.8S ribosomal DNA region was sequenced for asexual, anthropophilic dermatophyte species with morphological similarity to Trichophyton rubrum, as well as for members of the three previously delineated, related major clades in the T. mentagrophytes complex. Representative isolates of T. raubitschekii, T. fischeri, and T. kanei were found to have ITS sequences identical to that of T. rubrum. The ITS sequences of T. soudanense and T. megninii differed from that of T. rubrum by only a small number of base pairs. Their continued status as species, however, appears to meet criteria outlined in the population genetics-based cohesion species concept of A. R. Templeton. The ITS sequence of T. tonsurans differed from that of the biologically distinct T. equinum by only 1 bp, while the ITS sequence of the recently described species T. krajdenii had a sequence identical to that of T. mentagrophytes isolates related to the teleomorph Arthroderma vanbreuseghemii.

* Corresponding author. Mailing address: Mycology Laboratory, Laboratories Branch, Ontario Ministry of Health, 81 Resources Rd., Toronto, Ontario M9P 3T1, Canada. Phone: (416) 235-5719. Fax: (416) 235-5951. E-mail: summerri{at}mail1.moh.gov.on.ca.

Journal of Clinical Microbiology, December 1999, p. 4005-4011, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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