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Journal of Clinical Microbiology, December 1999, p. 4034-4038, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Demonstration of Bartonella grahamii DNA in Ocular Fluids of a Patient with Neuroretinitis

F. T. Kerkhoff,1,* A. M. C. Bergmans,2 A. van der Zee,2 and A. Rothova1

Department of Ophthalmology, F. C. Donders Institute, University Hospital, Utrecht,1 and Laboratory of Molecular Microbiology, St. Elisabeth Hospital, Tilburg,2 The Netherlands

Received 26 April 1999/Returned for modification 4 June 1999/Accepted 24 August 1999

We describe the clinical and laboratory features of a 55-year-old human immunodeficiency virus-negative female patient who presented with bilateral intraocular inflammatory disease (neuroretinitis type) and behavioral changes caused by a Bartonella grahamii infection. Diagnosis was based on the PCR analysis of DNA extracted from the intraocular fluids. DNA analysis of the PCR product revealed a 100% identity with the 16S rRNA gene sequence of B. grahamii. The patient was successfully treated with doxycycline (200 mg/day) and rifampin (600 mg/day) for 4 weeks. This is the first report that demonstrates the presence of a Bartonella species in the intraocular fluids of a nonimmunocompromised patient and that indicates that B. grahamii is pathogenic for humans.

* Corresponding author. Mailing address: Department of Ophthalmology, F. C. Donders Institute, Academic Hospital Utrecht, P.O. Box 85 500, 3508 GA Utrecht, The Netherlands. Phone: (30) 2507880. Fax: (30) 250 54 17. E-mail: fkerkh{at}oogheel.azu.nl.

Journal of Clinical Microbiology, December 1999, p. 4034-4038, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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