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Journal of Clinical Microbiology, December 1999, p. 4086-4092, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Scored Antibody Reactivity Determined by Immunoblotting Shows an Association between Clinical Manifestations and Presence of Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, and B. Valaisiana in Humans

Karine Ryffel,1 Olivier Péter,1,* Bernard Rutti,2 André Suard,3 and Eric Dayer1

Maladies Infectieuses et Immunologie, Institut Central des Hôpitaux Valaisans, 1950 Sion-CH,1 Institut de Zoologie, Université de Neuchâtel, 2007 Neuchâtel-CH,2 and Rue du Bourg 1, 1870 Monthey,3 Switzerland

Received 1 March 1999/Returned for modification 3 May 1999/Accepted 16 August 1999

An immunoglobulin G immunoblot was developed with antigenic extracts of Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, and B. valaisiana genospecies and was reacted with sera from patients with neuroborreliosis, acrodermatitis, and Lyme arthritis. A detailed analysis of the reactivities of the protein bands was performed, and a two-step scoring procedure was selected to determine the preferential reactivity of sera to one particular genospecies. The discriminative potential of 5 proteins (12-kDa, 16-kDa, 18-kDa, OspA, and 66-kDa proteins) was used as a rapid first-step scoring method, followed by scoring of 14 additional protein bands if necessary. The advantage of this procedure is the low percentage of serum samples with inconclusive results for one of the four species (10% for patients with neuroborreliosis, 6% for patients with acrodermatitis chronica atrophicans, and 6% for patients with Lyme arthritis). Among 31 serum samples from patients with neuroborreliosis, 16 were more reactive to B. garinii, 7 were more reactive to B. afzelii, 3 were more reactive to B. valaisiana, and 2 were more reactive to B. burgdorferi sensu stricto. Of 31 serum samples from patients with acrodermatitis, 26 showed a higher level of reactivity to B. afzelii. Of 34 serum samples from patients with Lyme arthritis, 21 were more reactive to B. burgdorferi sensu stricto, 10 were more reactive to B. afzelii, and 1 was more reactive to B. valaisiana. Our results suggest an organotropism of Borrelia species and provide some evidence of a pathogenic potential of B. valaisiana in humans.

* Corresponding author. Mailing address: Maladies Infectieuses et Immunologie, Institut Central des Hôpitaux Valaisans, Av. Grand-Champsec, CH-1950 Sion, Switzerland. Phone: 41 27 603 47 90. Fax: 41 27 603 48 93. E-mail: olivier.peter{at}ichv.vsnet.ch.

Journal of Clinical Microbiology, December 1999, p. 4086-4092, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.