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Journal of Clinical Microbiology, December 1999, p. 4135-4138, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differentiation of Two Biovars of Ureaplasma urealyticum Based on the 16S-23S rRNA Intergenic Spacer Region

Ryô Harasawa1,* and Yasuo Kanamoto2

Animal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033,1 and Department of Nutrition, Hiroshima Chuo Women's Junior College, Asaminami-ku, Hiroshima 731-0138,2 Japan

Received 9 June 1999/Returned for modification 21 July 1999/Accepted 20 August 1999

The 16S-23S rRNA intergenic spacer regions of 14 strains representing the 14 serovars of Ureaplasma urealyticum were amplified by PCR and sequenced for genetic differentiation between the two biovars Parvo and T960. Although the spacer region of the Parvo and T960 biovars comprised 302 nucleotides and lacked spacer tRNA genes, 15 nucleotides were different between the two biovars. The four nucleotide sequences of the 16S-23S rRNA intergenic spacer region of serovars 1, 3, 6, and 14 in the Parvo biovar were found to be identical. Similarly, the 10 nucleotide sequences of the 16S-23S rRNA intergenic spacer region of serovars 2, 4, 5, and 7 to 13 in the T960 biovar were found to be identical. The nucleotide sequence of the T960 biovar contains multiple restriction sites for restriction endonuclease SspI, which allows differentiation of the T960 biovar from the Parvo biovar.

* Corresponding author. Mailing address: Animal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81-3-5841-3626. Fax: 81-3-5841-3679. E-mail: ryo{at}m.u-tokyo.ac.jp.

Journal of Clinical Microbiology, December 1999, p. 4135-4138, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Pitcher, D., Sillis, M., Robertson, J. A. (2001). Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR-Single-Strand Conformation Polymorphism Analysis. J. Clin. Microbiol. 39: 1840-1844 [Abstract] [Full Text]  


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