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Journal of Clinical Microbiology, December 1999, p. 4139-4141, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Virusesdagger

K. Williams,Dagger S. Blake, A. Sweeney,§ J. T. Singer, and B. L. Nicholson*

Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, Maine 04469

Received 8 April 1999/Returned for modification 9 July 1999/Accepted 31 August 1999

A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.

* Corresponding author. Mailing address: Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, ME 04469. Phone: (207) 581-2800. Fax: (207) 581-2801. E-mail: brucen{at}maine.maine.edu.

dagger Maine Agricultural and Forest Experiment Station publication no. 2369.

Dagger Present address: DNA Sequencing Facility, University of Maine, Orono, ME 04469-5735.

§ Present address: Department of Dermatology, University of North Carolina, Chapel Hill, NC 27599.

Journal of Clinical Microbiology, December 1999, p. 4139-4141, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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