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Journal of Clinical Microbiology, December 1999, p. 4158-4160, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene

Stephen M. Marshall,1 Pasquale L. Melito,2 David L. Woodward,2 Wendy M. Johnson,2 Frank G. Rodgers,2 and Michael R. Mulvey2,*

Canadian Science Centre for Human and Animal Health, Bureau of Microbiology/Laboratory Center for Disease Control, Winnipeg, Manitoba R3E 3R2,2 and National Research Council Canada, Institute for Biological Sciences, Ottawa, Ontario K1A 0R6,1 Canada

Received 30 March 1999/Returned for modification 4 June 1999/Accepted 19 August 1999

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified by this assay.

* Corresponding author. Mailing address: Canadian Science Centre for Human and Animal Health, Bureau of Microbiology/Laboratory Center for Disease Control, 1015 Arlington St., Winnipeg, Manitoba R3E 3R2, Canada. Phone: (204) 789-2133. Fax: (204) 789-2018. E-mail: Michael_Mulvey{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, December 1999, p. 4158-4160, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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