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Journal of Clinical Microbiology, December 1999, p. 4163-4166, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Posttreatment Follow-Up of Brucellosis by PCR
Assay
Pilar
Morata,1
María Isabel
Queipo-Ortuño,1
José
María
Reguera,2
Miguel
Angel
García-Ordoñez,2
Cristina
Pichardo,3 and
Juan de Dios
Colmenero2,*
Department of Biochemistry and Molecular
Biology, Faculty of Medicine, University of
Malaga,1 and Infectious Diseases Unit,
Department of Internal Medicine, "Carlos Haya"
Hospital,2 Malaga, and Infectious
Diseases Service, "Virgen del Rocío" University Hospital,
Seville,3 Spain
Received 14 May 1999/Returned for modification 3 July 1999/Accepted 21 August 1999
In order to evaluate the usefulness of a peripheral blood PCR assay
in the posttreatment follow-up of brucellosis, a cohort of 30 patients
was studied by means of blood cultures, rose Bengal, seroagglutination,
Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at
the end of treatment, and 2, 4, and 6 months later. Of the 29 patients
whose PCR assays were initially positive, 28 (96.5%) were negative at
the conclusion of the treatment. PCR was positive for the two patients
who had relapses and negative for another four who had suspected but
unconfirmed relapses. PCR was negative for 98.3% of the follow-up
samples from those patients who had a favorable evolution. In
conclusion, PCR appears to be a very useful technique, not only for the
initial diagnosis of the disease, but also for posttreatment follow-up
and the early detection of relapses.
*
Corresponding author. Mailing address: Unidad de
Enfermedades Infecciosas, Dpto de Medicina Interna, Complejo
Hospitalario Carlos Haya, Camino de Antequera s/n, 29010 Malaga, Spain.
Phone: 34 5 2645809. Fax: 34 5 2645755. E-mail:
colmene{at}interbook.net.
Journal of Clinical Microbiology, December 1999, p. 4163-4166, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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