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Journal of Clinical Microbiology, December 1999, p. 4163-4166, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Posttreatment Follow-Up of Brucellosis by PCR Assay

Pilar Morata,1 María Isabel Queipo-Ortuño,1 José María Reguera,2 Miguel Angel García-Ordoñez,2 Cristina Pichardo,3 and Juan de Dios Colmenero2,*

Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Malaga,1 and Infectious Diseases Unit, Department of Internal Medicine, "Carlos Haya" Hospital,2 Malaga, and Infectious Diseases Service, "Virgen del Rocío" University Hospital, Seville,3 Spain

Received 14 May 1999/Returned for modification 3 July 1999/Accepted 21 August 1999

In order to evaluate the usefulness of a peripheral blood PCR assay in the posttreatment follow-up of brucellosis, a cohort of 30 patients was studied by means of blood cultures, rose Bengal, seroagglutination, Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at the end of treatment, and 2, 4, and 6 months later. Of the 29 patients whose PCR assays were initially positive, 28 (96.5%) were negative at the conclusion of the treatment. PCR was positive for the two patients who had relapses and negative for another four who had suspected but unconfirmed relapses. PCR was negative for 98.3% of the follow-up samples from those patients who had a favorable evolution. In conclusion, PCR appears to be a very useful technique, not only for the initial diagnosis of the disease, but also for posttreatment follow-up and the early detection of relapses.

* Corresponding author. Mailing address: Unidad de Enfermedades Infecciosas, Dpto de Medicina Interna, Complejo Hospitalario Carlos Haya, Camino de Antequera s/n, 29010 Malaga, Spain. Phone: 34 5 2645809. Fax: 34 5 2645755. E-mail: colmene{at}interbook.net.

Journal of Clinical Microbiology, December 1999, p. 4163-4166, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.