This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morata, P.
Right arrow Articles by Colmenero, J. d. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morata, P.
Right arrow Articles by Colmenero, J. d. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 1999, p. 4163-4166, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Posttreatment Follow-Up of Brucellosis by PCR Assay

Pilar Morata,1 María Isabel Queipo-Ortuño,1 José María Reguera,2 Miguel Angel García-Ordoñez,2 Cristina Pichardo,3 and Juan de Dios Colmenero2,*

Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Malaga,1 and Infectious Diseases Unit, Department of Internal Medicine, "Carlos Haya" Hospital,2 Malaga, and Infectious Diseases Service, "Virgen del Rocío" University Hospital, Seville,3 Spain

Received 14 May 1999/Returned for modification 3 July 1999/Accepted 21 August 1999

In order to evaluate the usefulness of a peripheral blood PCR assay in the posttreatment follow-up of brucellosis, a cohort of 30 patients was studied by means of blood cultures, rose Bengal, seroagglutination, Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at the end of treatment, and 2, 4, and 6 months later. Of the 29 patients whose PCR assays were initially positive, 28 (96.5%) were negative at the conclusion of the treatment. PCR was positive for the two patients who had relapses and negative for another four who had suspected but unconfirmed relapses. PCR was negative for 98.3% of the follow-up samples from those patients who had a favorable evolution. In conclusion, PCR appears to be a very useful technique, not only for the initial diagnosis of the disease, but also for posttreatment follow-up and the early detection of relapses.

* Corresponding author. Mailing address: Unidad de Enfermedades Infecciosas, Dpto de Medicina Interna, Complejo Hospitalario Carlos Haya, Camino de Antequera s/n, 29010 Malaga, Spain. Phone: 34 5 2645809. Fax: 34 5 2645755. E-mail: colmene{at}interbook.net.

Journal of Clinical Microbiology, December 1999, p. 4163-4166, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Castano, M. J., Solera, J. (2009). Chronic Brucellosis and Persistence of Brucella melitensis DNA. J. Clin. Microbiol. 47: 2084-2089 [Abstract] [Full Text]  
  • Casanova, A., Ariza, J., Rubio, M., Masuet, C., Diaz, R. (2009). BrucellaCapt versus Classical Tests in the Serological Diagnosis and Management of Human Brucellosis. CVI 16: 844-851 [Abstract] [Full Text]  
  • MAAS, K. S. J. S. M., MENDEZ, M., ZAVALETA, M., MANRIQUE, J., FRANCO, M. P., MULDER, M., BONIFACIO, N., CASTANEDA, M. L., CHACALTANA, J., YAGUI, E., GILMAN, R. H., GUILLEN, A., BLAZES, D. L., ESPINOSA, B., HALL, E., ABDOEL, T. H., SMITS, H. L., THE BRUCELLOSIS WORKING GROUP IN CALLAO, (2007). EVALUATION OF BRUCELLOSIS BY PCR AND PERSISTENCE AFTER TREATMENT IN PATIENTS RETURNING TO THE HOSPITAL FOR FOLLOW-UP. Am J Trop Med Hyg 76: 698-702 [Abstract] [Full Text]  
  • Mitka, S., Anetakis, C., Souliou, E., Diza, E., Kansouzidou, A. (2007). Evaluation of Different PCR Assays for Early Detection of Acute and Relapsing Brucellosis in Humans in Comparison with Conventional Methods. J. Clin. Microbiol. 45: 1211-1218 [Abstract] [Full Text]  
  • Pappas, G., Akritidis, N., Bosilkovski, M., Tsianos, E. (2005). Brucellosis. NEJM 352: 2325-2336 [Full Text]  
  • Morata, P., Queipo-Ortuno, M. I., Reguera, J. M., Garcia-Ordonez, M. A., Cardenas, A., Colmenero, J. D. (2003). Development and Evaluation of a PCR-Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Brucellosis. J. Clin. Microbiol. 41: 144-148 [Abstract] [Full Text]  
  • Bardenstein, S., Mandelboim, M., Ficht, T. A., Baum, M., Banai, M. (2002). Identification of the Brucella melitensis Vaccine Strain Rev.1 in Animals and Humans in Israel by PCR Analysis of the PstI Site Polymorphism of Its omp2 Gene. J. Clin. Microbiol. 40: 1475-1480 [Abstract] [Full Text]  
  • Morata, P., Queipo-Ortuno, M. I., Reguera, J. M., Miralles, F., Lopez-Gonzalez, J. J., Colmenero, J. D. (2001). Diagnostic Yield of a PCR Assay in Focal Complications of Brucellosis. J. Clin. Microbiol. 39: 3743-3746 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»