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Journal of Clinical Microbiology, December 1999, p. 4183-4185, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of a Rapid Assay for Detection of Chlamydia trachomatis Infections in Outpatient Clinics in South Kalimantan, Indonesia

Susana Widjaja,1 Surekha Cohen,2 William E. Brady,3 Kevin O'reilly,4 Susanto,5 Ajar Wibowo,6 Cahyono,7 Robert R. Graham,1 and Kevin R. Porter1,*

Viral Diseases Program, U.S. Naval Medical Research Unit No. 2,1 and MotherCare,2 Jakarta, and Ulin Provencial Hospital,5 Banjar Baru District Hospital,6 and Kandangan District Hospital,7 Kalimantan, Indonesia; MotherCare, Washington, D.C.3; and Division of Reproductive Tract, World Health Organization, Switzerland4

Received 15 April 1999/Returned for modification 8 June 1999/Accepted 20 September 1999

A multicenter cross-sectional survey was conducted comparing a commercially available chlamydial optical immunoassay (OIA) to the chlamydial ligase chain reaction (LCR). Endocervical samples from 415 outpatients visiting clinics from three hospitals in South Kalimantan, Indonesia, were evaluated. Relative to the LCR, the overall sensitivity and specificity of the OIA were 31.6 and 98.9%, respectively. The sensitivity of the OIA varied among the three hospital laboratories, ranging from 20 to 50%. The OIA performance was slightly lower on samples from patients attending dermatovenereology clinics than on samples from nondermatovenereology clinic patients. The results indicate that the OIA did not perform well compared to LCR.

* Corresponding author. Mailing address: Virus Research Program, U.S. Naval Medical Research Unit No. 2, Unit 8132, APO AP95620-8132. Phone: 62-21-4214457. Fax: 62-21-4244507. E-mail: porterkr{at}namru2.com.

Journal of Clinical Microbiology, December 1999, p. 4183-4185, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.